School of Biology and Ecology, University of Maine, Orono, ME 04469, USA.
J Econ Entomol. 2009 Dec;102(6):2078-83. doi: 10.1603/029.102.0609.
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most damaging insect pests of potato, Solanum tuberosum L. Novaluron is a relatively new benzoylphenyl urea insect growth regulator with good activity against this pest. Earlier studies revealed that feeding on potato foliage treated with novaluron induces reversible egg hatch inhibition in adult Colorado potato beetles. We investigated whether novaluron effects depend on physiological state of the beetles at the time of exposure. The following four treatments were created: young beetles unmated at the beginning of the experiment and feeding on potato foliage treated with novaluron, young beetles unmated at the beginning of the experiment and feeding on untreated foliage, older beetles mated at the beginning of the experiment and feeding on foliage treated with novaluron, and older beetles mated at the beginning of the experiment and feeding on untreated foliage. The beetles were exposed to the respective treatments for 5 d. After that, both young and older beetles feeding on novaluron-treated leaves were switched onto untreated leaves and monitored for another 5 d to test their ability to recover. Young beetles unmated at the beginning of the experiment produced more eggs after feeding on the treated foliage, possibly indicating the presence of a pesticide-induced homeostatic modulation. No such effect was observed in the older beetles. Regardless of beetle physiological state at the beginning of the experiment, eggs produced on treated foliage did not hatch. The beetles eventually resumed laying viable eggs after being switched onto untreated foliage, with the recovery being delayed by approximately 24 h in young beetles compared with older beetles. Our results corroborate that novaluron reduces fertility of treated adults.
科罗拉多马铃薯甲虫，Leptinotarsa decemlineata（Say），是马铃薯，Solanum tuberosum L. 的最具破坏性的害虫之一。Novaluron 是一种相对较新的苯甲酰基苯甲脒昆虫生长调节剂，对这种害虫具有良好的活性。早期的研究表明，取食经 novaluron 处理的马铃薯叶片会诱导成年科罗拉多马铃薯甲虫的卵孵化可逆抑制。我们研究了 novaluron 的作用是否取决于暴露时甲虫的生理状态。创建了以下四种处理方法：实验开始时未交配的年轻甲虫和取食经 novaluron 处理的马铃薯叶片，实验开始时未交配的年轻甲虫和取食未经处理的叶片，实验开始时交配的年长甲虫和取食经 novaluron 处理的叶片，以及实验开始时交配的年长甲虫和取食未经处理的叶片。甲虫暴露于各自的处理 5 天。之后，取食经 novaluron 处理叶片的年轻和年长甲虫均切换至未经处理的叶片，并监测 5 天，以测试其恢复能力。实验开始时未交配的年轻甲虫在取食处理后的叶片后产生更多的卵，这可能表明存在杀虫剂诱导的体内平衡调节。年长甲虫则未观察到这种效应。无论实验开始时甲虫的生理状态如何，在处理叶片上产下的卵都不会孵化。甲虫最终在切换到未经处理的叶片后恢复产卵，并且与年长甲虫相比，年轻甲虫的恢复延迟了约 24 小时。我们的结果证实 novaluron 降低了处理成虫的生育能力。
