USDA-ARS, Plant Genetics Research Unit,University of Missouri, Columbia, MO 65211, USA.
J Econ Entomol. 2009 Dec;102(6):2350-9. doi: 10.1603/029.102.0642.
Seven maize, Zea mays L., genotypes selected for native resistance to western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), larval feeding damage (SUM2068, SUM2162, CRW3(S1)C6, NSS1 x CRW3(S1)C6, PI583927, CRW2(C5), and AR17056-16) were evaluated along with three control maize genotypes in the field for plant damage, larval recovery, adult emergence, root size, and root regrowth. Larvae recovered were further analyzed for head capsule width and dry weight and adults for dry weight. All factors evaluated with the exception of adult dry weight varied significantly among maize genotypes. Control genotypes included a highly susceptible hybrid, B37 x H84, a transgenic rootworm-resistant hybrid expressing the modified Cry3A protein (MIR604), and the untransformed modern hybrid with the same genetic background as the MIR604 we used (isoline) as a second susceptible control. In general, the genotypes previously selected for resistance to western corn rootworm larval feeding had less damage, fewer larvae recovered, smaller larvae recovered, and fewer adults recovered than the susceptible controls. SUM2162 was significantly less damaged than all other native sources of resistance. Western corn rootworm larvae recovered from SUM2162 and SUM2068 were significantly smaller in terms of head capsule width and average weight than larvae recovered from all other maize genotypes, indicating that antibiosis is a mechanism of resistance for these two hybrids.
七种玉米，Zea mays L.，经过选择具有对西部玉米根虫，Diabrotica virgifera virgifera LeConte（鞘翅目：金龟子科）幼虫取食损伤的本地抗性，用于幼虫取食损伤（SUM2068、SUM2162、CRW3(S1)C6、NSS1 x CRW3(S1)C6、PI583927、CRW2(C5)和 AR17056-16），以及三种对照玉米基因型进行了田间评价，评估了植株损伤、幼虫回收、成虫出现、根系大小和根系再生。回收的幼虫进一步分析了头壳宽度和干重，成虫分析了干重。除了成虫干重外，所有评估因素在玉米基因型之间均有显著差异。对照基因型包括一个高度易感的杂交种 B37 x H84、表达改良 Cry3A 蛋白的转基因根虫抗性杂交种（MIR604），以及与我们使用的 MIR604 具有相同遗传背景的未转化现代杂交种（同系物）作为第二个易感对照。一般来说，先前选择对西部玉米根虫幼虫取食具有抗性的基因型比易感对照的损伤小、回收的幼虫少、回收的幼虫小、回收的成虫少。SUM2162 的损伤明显小于所有其他本地抗性来源。从 SUM2162 和 SUM2068 回收的西部玉米根虫幼虫在头壳宽度和平均重量方面明显小于从所有其他玉米基因型回收的幼虫，表明这些两种杂交种的抗生性是一种抗性机制。
