He Juanmei, Zhu Jun, Chao Guoxiang, Zhu Xiaofang, Zhu Xiaoping, Dong Lanmei, Yang Weixia, Zhu Guoqiang
College of Veterinary medicine, Yangzhou University, Yangzhou 225009, China.
Wei Sheng Wu Xue Bao. 2009 Oct;49(10):1397-402.
Staphylococcus aureus small colony variants (S. aureus SCVs) could lead to persistent, recurrent infection with the characteristics of aminoglycosides antibiotics resistance, making them a big challenge for clinical diagnosis and therapy. We aimed at isolating and identifying isolates of S. aureus SCVs and providing the biological material of SCVs study in China.
The combination assays of observing colony phenotype, identification of the species-specific gene nuc of S. aureus by PCR amplification and a series of biochemical tests were conducted on 104 clinical isolates originally isolated from human, cow and environment. The suspected isolates were confirmed as S. aureus SCVs by complementation assay with supplementation of menadione, thiamine, thymine and haemin.
One of the isolates from environment was identified as SCVs, named CDC54 with the species-specific gene nuc of Staphylococcus aureus (S. aureus) confirmed by PCR amplification, whose major phenotypes included smaller colony, decreased pigmentation, decreased coagulase, reduced fermentation of lactose, decreased haemolytic activity, increased resistance to aminoglycosides.
The CDC54 will play an important role in studying prevention, control and pathogenesis for S. aureus SCVs infection.
金黄色葡萄球菌小菌落变异株（S. aureus SCVs）可导致持续性、复发性感染，具有对氨基糖苷类抗生素耐药的特点，给临床诊断和治疗带来巨大挑战。我们旨在分离和鉴定金黄色葡萄球菌小菌落变异株，并提供中国SCVs研究的生物材料。
对最初从人、牛和环境中分离出的104株临床分离株进行观察菌落表型、通过PCR扩增鉴定金黄色葡萄球菌种特异性基因nuc以及一系列生化试验的联合检测。通过补充甲萘醌、硫胺素、胸腺嘧啶和血红素的互补试验，将疑似分离株确认为金黄色葡萄球菌小菌落变异株。
从环境中分离出的一株被鉴定为SCVs，命名为CDC54，通过PCR扩增确认其具有金黄色葡萄球菌（S. aureus）的种特异性基因nuc，其主要表型包括菌落较小、色素沉着减少、凝固酶减少、乳糖发酵减少、溶血活性降低、对氨基糖苷类耐药性增加。
CDC54将在金黄色葡萄球菌小菌落变异株感染的预防、控制和发病机制研究中发挥重要作用。
