SPSS 正交设计在金线莲组织培养中的应用

[Application of SPSS orthogonal design in tissue culture of Anoectochilus roxburghii].

作者信息

Zhang Fusheng, Guo Shunxing

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2581-5.

PMID:20069896

Abstract

OBJECTIVE

To study the effect of the different constitutions of plant hormone on the development of Anoectochilus roxburghii.

METHOD

A. roxburghii were harvested after having been cultured for 60 days. An orthogonal design was used to study the effect of NAA and 6-BA on the leaf number, eustipe number, lateral branch number of the stem tip and stem section, and the height of the stem tips. All of the data were processed by SPSS.

RESULT AND CONCLUSION

It is reported for the first time that NAA could make different development of A. roxburghii at low concentration ( < 1 mg L(-1)) and high concentration ( > 1 mg L(-1)). The optimum constitution of MS medium was NAA 0.5 mg L(-1) + 6-BA 1 mg L(-1) for the growth of the stem tip of A. roxburghii, and NAA 1 mg L(-1) + 6-BA 2 mg L(-1) for the differentiation of bud and the formation of lateral branch of the stem section. The different concentrations of NAA and 6-BA had different effects on the growth and differentiation of the stem tip and the stem section of A. roxburghii.

摘要

目的

研究不同植物激素组合对金线莲生长发育的影响。

方法

培养60天后采收金线莲。采用正交设计研究萘乙酸（NAA）和6-苄氨基腺嘌呤（6-BA）对金线莲叶片数、叶柄数、茎尖和茎段侧枝数以及茎尖高度的影响。所有数据均用SPSS软件处理。

结果与结论

首次报道低浓度（<1 mg L⁻¹）和高浓度（>1 mg L⁻¹）的NAA对金线莲生长发育有不同影响。对于金线莲茎尖生长，MS培养基的最佳组合为NAA 0.5 mg L⁻¹ + 6-BA 1 mg L⁻¹；对于茎段芽分化和侧枝形成，最佳组合为NAA 1 mg L⁻¹ + 6-BA 2 mg L⁻¹。不同浓度的NAA和6-BA对金线莲茎尖和茎段的生长及分化有不同影响。