不同栽培阶段金线莲的非靶向代谢组学分析

[Nontargeted metabolomic analysis of Anoectochilus roxburghii at different cultivation stages].

作者信息

Li Rui-Zi, Lin Jun, Wang Xia-Xia, Yu Xiao-Min, Chen Can-Li, Guan Yue-Feng

机构信息

College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, China.

Horticultural Plant Biology and Metabolomics Center, Haixia Institute of Science and Technology, Fujian Agricultural and Forestry University, Fuzhou 350002, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2017 Dec;42(23):4624-4630. doi: 10.19540/j.cnki.cjcmm.20170928.007.

DOI:10.19540/j.cnki.cjcmm.20170928.007

PMID:29376262

Abstract

Anoectochilus roxburghii is a traditional Chinese medicine and natural health products. In the modern cultivation system, A. roxburghii is micropropagated in tissue culture, and the plants are transferred to soil cultivation for months. However, it remains unclear about the necessity of soil cultivation for the accumulation of health beneficial compounds. In this paper, we performed nontargeted metabolomic analysis using GC-TOF-MS and UPLC-Q-TOF-MS, on A. roxburghii plants at tissue culture stage or after 3 months of soil cultivation. The results showed that the primary metabolites such as alcohols and organic acids are abundant in the tissue culture plants. In contrast, polysaccharide, nucleoside, esters and secondary metabolites such as flavonoids, terpenoids were significantly accumulated in cultivated seedlings. Flavonoids and polysaccharides are considered as the principle effective components in A. roxburghii. Soil cultivation period is therefore essential for the accumulation of these metabolites.

摘要

金线莲是一种传统中药和天然保健品。在现代栽培体系中，金线莲通过组织培养进行微繁殖，然后将植株转移到土壤中栽培数月。然而，对于土壤栽培对于有益健康化合物积累的必要性仍不清楚。在本文中，我们使用气相色谱-飞行时间质谱（GC-TOF-MS）和超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对处于组织培养阶段或土壤栽培3个月后的金线莲植株进行了非靶向代谢组学分析。结果表明，在组织培养植株中，醇类和有机酸等初级代谢产物含量丰富。相比之下，多糖、核苷、酯类以及黄酮类、萜类等次级代谢产物在栽培幼苗中显著积累。黄酮类和多糖被认为是金线莲的主要有效成分。因此，土壤栽培期对于这些代谢产物的积累至关重要。