Khalidi N, McKenzie I, McKenzie J K
Department of Medicine, University of Manitoba, Winnipeg, Canada.
Am J Hypertens. 1991 Jan;4(1 Pt 1):56-9. doi: 10.1093/ajh/4.1.56.
Using flat bed isoelectric focusing, inactive renins from various body fluids were compared. Normal plasma inactive renin demonstrated six consistent peaks at pH 4.98, 5.14, 5.29, 5.47, 5.63 and 5.91, the largest being 5.29, 5.47 and 5.63. The isoelectric pattern of ovarian follicular fluid inactive renin was similar, with slight shifts at major peaks to a higher pH. Pregnancy plasma had inactive renin patterns like normal human plasma as did pleural fluid, lymphocoele fluid and plasma from anephric humans. Amniotic fluid inactive renin showed a markedly different pattern with major peaks at pH 5.00, 5.16 and 5.30. Fetal plasma (cord blood) also showed differences with only four peaks at 5.24, 5.40, 5.58 and 5.83. We conclude that human prorenin isoelectric patterns are similar for plasma, ovarian and most tissue fluid prorenins, but different for fetal plasma and amniotic fluid suggesting that these forms of renin do not cross the placental barrier.
利用平板等电聚焦技术，对来自各种体液的无活性肾素进行了比较。正常血浆中的无活性肾素在pH 4.98、5.14、5.29、5.47、5.63和5.91处显示出六个一致的峰，其中最大的峰在5.29、5.47和5.63处。卵巢卵泡液中无活性肾素的等电模式相似，主要峰处有轻微向更高pH值的偏移。妊娠血浆中的无活性肾素模式与正常人血浆相似，胸膜液、淋巴管囊肿液和无肾者的血浆也是如此。羊水无活性肾素呈现出明显不同的模式，主要峰在pH 5.00、5.16和5.30处。胎儿血浆（脐带血）也显示出差异，仅在5.24、5.40、5.58和5.83处有四个峰。我们得出结论，人肾素原的等电模式对于血浆、卵巢和大多数组织液中的肾素原是相似的，但对于胎儿血浆和羊水则不同，这表明这些形式的肾素不会穿过胎盘屏障。
