Matinlauri I, Eskola J U, Aalto M, Koskinen P, Irjala K
Department of Clinical Chemistry, Turku University Central Hospital, Finland.
Clin Chem. 1994 Jan;40(1):74-9.
We developed a new time-resolved immunofluorometric assay (TR-IFMA) using two monoclonal antibodies for the total renin measurement in human plasma and follicular fluid. No conversion of prorenin to renin was needed because the assay detected both renin and prorenin. The detection limit of the assay was 10 ng/L and the linear range was 10-25,000 ng/L. Within-assay precision (CV) was 15-8% at renin concentrations of 50-12,200 ng/L. Between-assay precision was 19-3% at concentrations of 100-18,000 ng/L. Analytical recovery of added renin was 85-104% (n = 5) in plasma samples and 104-119% (n = 3) in follicular fluids. For plasma, the reference interval was 78-262 ng/L in men (n = 44) and 36-226 ng/L in women (n = 43).
我们开发了一种新的时间分辨免疫荧光分析法（TR-IFMA），该方法使用两种单克隆抗体来检测人血浆和卵泡液中的总肾素。由于该检测方法可同时检测肾素和前肾素，因此无需将前肾素转化为肾素。该检测方法的检测限为10 ng/L，线性范围为10 - 25,000 ng/L。在肾素浓度为50 - 12,200 ng/L时，批内精密度（CV）为15 - 8%。在浓度为100 - 18,000 ng/L时，批间精密度为19 - 3%。添加肾素在血浆样本中的分析回收率为85 - 104%（n = 5），在卵泡液中的分析回收率为104 - 119%（n = 3）。对于血浆，男性（n = 44）的参考区间为78 - 262 ng/L，女性（n = 43）的参考区间为36 - 226 ng/L。
