Stajner A, Sůva J
J Clin Chem Clin Biochem. 1977 Sep;15(9):513-4. doi: 10.1515/cclm.1977.15.1-12.513.
A modification of the method for the determination of nonesterified fatty acids in blood serum using a stable cupric reagent stabilized with sodium citrate, has been developed. 33 g of NaCl are dissolved in 100 ml of the described reagent, and its pH should not be additionally adjusted. The reagent is stable for at least one year if kept in the refrigerator at 5 degrees C. The determination may be done in 0.1--0.2 ml of blood serum. The relationship between the absorbance and concentration of palmitic acid is still linear at a concentration of 2500 mumol/l. Error of the method is +/- 3%.
已开发出一种用柠檬酸钠稳定的稳定铜试剂测定血清中非酯化脂肪酸的方法的改进方法。将33 g氯化钠溶解在100 ml所述试剂中，其pH值无需额外调节。如果保存在5摄氏度的冰箱中，该试剂至少稳定一年。测定可在0.1--0.2 ml血清中进行。在2500 μmol/l的浓度下，棕榈酸的吸光度与浓度之间的关系仍呈线性。该方法的误差为±3%。
