采用萃取-光度法测定血清游离脂肪酸。
Assay of serum free fatty acids by extraction-photometric procedure.
作者信息
Chromý V, Gergel J, Voznícek J, Krombholzová L, Musil J
出版信息
Clin Chim Acta. 1977 Oct 15;80(2):327-32. doi: 10.1016/0009-8981(77)90040-7.
Reaction conditions for extraction-photometric determination of free fatty acids in serum were studied. The results were found to be influenced by (a) sodium chloride concentration in the copper reagent, (b) the kind of standard solution used, (c) centrifugation of the reaction mixture and (d) serum phospholipids. A micromethod based on the formation of free fatty acids-copper soaps with a stable copper reagent and sensitive indicator for copper, 2-(2-thiazolylazo)-4-methoxyphenol, is described.
研究了血清中游离脂肪酸萃取光度测定的反应条件。结果发现受以下因素影响：(a) 铜试剂中氯化钠的浓度；(b) 所用标准溶液的种类；(c) 反应混合物的离心处理；(d) 血清磷脂。本文描述了一种基于游离脂肪酸与铜形成稳定的铜皂，并使用灵敏的铜指示剂2-(2-噻唑基偶氮)-4-甲氧基苯酚的微量方法。
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