Shah D, Kiran R, Wanchu A, Bhatnagar Archana
Department of Biochemistry, Basic Medical Science Block, Panjab University, Chandigarh, India.
Kathmandu Univ Med J (KUMJ). 2009 Jul-Sep;7(27):213-9. doi: 10.3126/kumj.v7i3.2726.
Systemic Lupus Erythematosus (SLE) is a complex chronic immunological disease characterized by increased B cell activity and altered T cell function.
To investigate relationship between T lymphocyte subsets and cortisol with the disease activity of systemic lupus erythematosus patients in North India.
The percentage of CD4(+) and CD8(+) T cells in the lymphocyte of SLE patients and healthy controls were determined by flow cytometry. Serum cortisol of SLE patients and healthy controls was determined by enzyme-linked immunosorbent assay (ELISA).
A significant decrease in the percentage of CD4(+) T cells and increase in the percentage of CD8(+) T cells were found in patients with SLE compared to the healthy controls. Decrease in the ratio of CD4(+)/CD8(+) T cell and low level of serum cortisol were found in the patients with SLE. The ratio of CD4(+)/CD8(+) T cell was inversely correlated with systemic lupus erythematosus disease activity index (SLEDAI) score and erythrocyte sedimentation rate (ESR). A positive correlation was observed between CD8(+) T cells and SLEDAI score. Furthermore, CD8(+) T cells were positively correlated with ESR in the patients with SLE.
The results showed that low level of cortisol and high percentage of CD8(+) T cells in the lymphocytes could be actively involved in the pathogenesis of SLE.
系统性红斑狼疮（SLE）是一种复杂的慢性免疫疾病，其特征为B细胞活性增加和T细胞功能改变。
研究印度北部系统性红斑狼疮患者的T淋巴细胞亚群和皮质醇与疾病活动度之间的关系。
采用流式细胞术测定SLE患者和健康对照淋巴细胞中CD4(+)和CD8(+) T细胞的百分比。采用酶联免疫吸附测定法（ELISA）测定SLE患者和健康对照的血清皮质醇。
与健康对照相比，SLE患者CD4(+) T细胞百分比显著降低，CD8(+) T细胞百分比升高。SLE患者CD4(+)/CD8(+) T细胞比值降低，血清皮质醇水平较低。CD4(+)/CD8(+) T细胞比值与系统性红斑狼疮疾病活动指数（SLEDAI）评分及红细胞沉降率（ESR）呈负相关。CD8(+) T细胞与SLEDAI评分呈正相关。此外，SLE患者中CD8(+) T细胞与ESR呈正相关。
结果表明，淋巴细胞中皮质醇水平低和CD8(+) T细胞百分比高可能积极参与SLE的发病机制。
