Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University School of Medicine, Medical Research Institute, Pusan National University, Busan, Korea.
Clin Exp Otorhinolaryngol. 2009 Dec;2(4):186-92. doi: 10.3342/ceo.2009.2.4.186. Epub 2009 Dec 31.
This study investigated the telomerase expression in peripheral blood mononuclear cells (PBMCs) and the relationship between the serum level of several soluble factors such as vascular endothelial growth factor (VEGF), hepatocyte growth factor, interleukin (IL)-6, IL-8, and matrix metallopeptidase-9 and the clinicopathological features of patients with head and neck squamous cell carcinoma (HNSCC).
Peripheral blood samples were collected from 50 HNSCC patients and 15 normal controls. The telomerase activity in the PBMCs was measured by Telomere Repeat Amplification Protocols. The serum levels of the soluble factors were analyzed by enzyme-linked immunosorbent assay.
The expression of telomerase in the PBMCs of HNSCC patients was significantly correlated with the N and American Joint Committee on Cancer (AJCC) stages. The serum VEGF level was significantly higher in the patients with an advanced T stage, N stage and AJCC stage. Serum VEGF was significantly related with the expression of telomerase in the PBMCs. The telomerase expression and the VEGF expression were shown to be independent factors associated with poor survival.
The telomerase expression in the PBMCs and the serum VEGF level of HNSCC patients were significantly correlated with the N stage, the AJCC stage and the prognosis.
本研究旨在探讨端粒酶在周围血单个核细胞（PBMCs）中的表达及其与血管内皮生长因子（VEGF）、肝细胞生长因子、白细胞介素（IL）-6、IL-8 和基质金属蛋白酶-9 等几种可溶性因子的血清水平与头颈部鳞状细胞癌（HNSCC）患者的临床病理特征之间的关系。
采集 50 例 HNSCC 患者和 15 例正常对照者外周血样本。采用端粒重复扩增协议（TRAP）测定 PBMC 中端粒酶活性。采用酶联免疫吸附试验（ELISA）分析可溶性因子的血清水平。
HNSCC 患者 PBMC 中端粒酶的表达与 N 分期和美国癌症联合委员会（AJCC）分期显著相关。T 分期、N 分期和 AJCC 分期较晚的患者血清 VEGF 水平显著升高。血清 VEGF 与 PBMC 中端粒酶的表达呈显著相关。端粒酶的表达和 VEGF 的表达均被证明是与预后不良相关的独立因素。
HNSCC 患者 PBMC 中端粒酶的表达及其血清 VEGF 水平与 N 分期、AJCC 分期和预后显著相关。
