Hathaway Bridget, Landsittel Douglas P, Gooding William, Whiteside Theresa L, Grandis Jennifer R, Siegfried Jill M, Bigbee William L, Ferris Robert L
Department of Otolaryngology, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.
Laryngoscope. 2005 Mar;115(3):522-7. doi: 10.1097/01.mlg.0000157850.16649.b8.
Our objective was to characterize cytokine profiles in the systemic circulation of patients with active squamous cell carcinoma of the head and neck (SCCHN) compared with long-term tobacco smokers as controls. Furthermore, we hypothesized that persistent immune dysregulation in patients cured of their disease may be reflected in altered cytokine profiles in the systemic circulation.
Retrospective, case-control study.
Using three well-defined clinical cohorts of SCCHN patients and smoker controls, we analyzed 100 microL of serum for a panel of 10 cytokines to determine whether serum cytokine profiles could distinguish clinically defined groups of SCCHN patients. Statistical analysis of multiplexed cytokine profiles was applied to classify three clinically defined groups: active SCCHN patients, treated SCCHN patients with no evidence of disease for over 3 years, and matched disease-free controls. Discrimination of outcome status was accomplished using classification trees, and 10-fold cross-validation was implemented to assess classification accuracy using independent data.
We show that multiplexed cytokine and chemokine profiling may be performed to reflect the immune status of SCCHN patients. Selected cytokine profiles indicate that immunologic responses to carcinogenesis may not normalize even in the absence of tumor for over 3 years.
Multiplexed serum cytokine profiles may be applicable to early detection, for screening those at high risk for SCCHN, and as clinically predictive biomarkers of disease status in successfully treated patients.
我们的目的是对活跃期头颈部鳞状细胞癌（SCCHN）患者的体循环中的细胞因子谱进行特征分析，并与作为对照的长期吸烟者进行比较。此外，我们假设疾病已治愈患者中持续存在的免疫失调可能反映在体循环中细胞因子谱的改变上。
回顾性病例对照研究。
我们使用三个定义明确的SCCHN患者临床队列和吸烟者对照，分析100微升血清中的一组10种细胞因子，以确定血清细胞因子谱是否能够区分临床定义的SCCHN患者组。对多重细胞因子谱进行统计分析，以对三个临床定义的组进行分类：活跃期SCCHN患者、治疗后3年以上无疾病证据的SCCHN患者以及匹配的无病对照。使用分类树完成结局状态的判别，并实施10倍交叉验证以使用独立数据评估分类准确性。
我们表明，可以进行多重细胞因子和趋化因子分析以反映SCCHN患者的免疫状态。选定的细胞因子谱表明，即使在无肿瘤超过3年的情况下，对致癌作用的免疫反应可能也不会恢复正常。
多重血清细胞因子谱可能适用于早期检测，用于筛查SCCHN高危人群，并作为成功治疗患者疾病状态的临床预测生物标志物。
