白细胞介素-32γ 通过激活 Erk1/2 增强成纤维样滑膜细胞中 IL-6 和 IL-8 的产生。
Interleukin-32gamma enhances the production of IL-6 and IL-8 in fibroblast-like synoviocytes via Erk1/2 activation.
机构信息
Division of Rheumatology, Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea.
出版信息
J Clin Immunol. 2010 Mar;30(2):260-7. doi: 10.1007/s10875-009-9360-2. Epub 2010 Jan 14.
INTRODUCTION
In the present study, we examined the effect of the pro-inflammatory cytokine IL-32gamma, the most biologically active isoform, and its related molecules in fibroblast-like synoviocytes (FLS).
MATERIALS AND METHODS
FLS were isolated from synovial tissues of rheumatoid arthritis (RA) patients. The secretion and expression of IL-6 and IL-8 were examined by ELISA and real-time PCR, and the activation of signaling molecules was evaluated by Western blot, electrophoretic mobility shift assay (EMSA), real-time PCR, and siRNA transfection.
RESULTS
By IL-32gamma stimulation in RA FLS, the expressions of IL-6 and IL-8 were increased significantly, and the phosphorylated Erk1/2 and AP-1 were expressed prominently in Western blot and EMSA. In the Erk1/2 inhibited cells, IL-32gamma stimulation did not increase the mRNA expression of IL-6 and IL-8.
CONCLUSION
Our results suggest that IL-32gamma stimulation can induce the production of IL-6 and IL-8 from RA FLS via Erk1/2 activation.
简介
在本研究中，我们研究了促炎细胞因子 IL-32gamma（最具生物活性的同种型）及其相关分子对成纤维样滑膜细胞（FLS）的影响。
材料与方法
从类风湿关节炎（RA）患者的滑膜组织中分离出 FLS。通过 ELISA 和实时 PCR 检测 IL-6 和 IL-8 的分泌和表达，通过 Western blot、电泳迁移率变动分析（EMSA）、实时 PCR 和 siRNA 转染评估信号分子的激活。
结果
在 RA FLS 中，IL-32gamma 刺激可显著增加 IL-6 和 IL-8 的表达，Western blot 和 EMSA 显示磷酸化 Erk1/2 和 AP-1 明显表达。在 Erk1/2 被抑制的细胞中，IL-32gamma 刺激并未增加 IL-6 和 IL-8 的 mRNA 表达。
结论
我们的结果表明，IL-32gamma 刺激可通过 Erk1/2 激活诱导 RA FLS 产生 IL-6 和 IL-8。
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