通过应用脂质衍生的多巴胺修饰加合物评估抗氧化食物因子的神经保护作用。

Assessing the neuroprotective effect of antioxidant food factors by application of lipid-derived dopamine modification adducts.

作者信息

Liu Xuebo, Yamada Naruomi, Osawa Toshihiko

机构信息

Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Science, Nagoya University, Nagoya, Japan.

出版信息

Methods Mol Biol. 2010;594:263-73. doi: 10.1007/978-1-60761-411-1_18.

DOI:10.1007/978-1-60761-411-1_18

PMID:20072923

Abstract

Advances in understanding the neurodegenerative pathologies are creating new opportunities for the development of neuroprotective therapies, such as antioxidant food factors, lifestyle modification and drugs. However, the biomarker by which the effect of the agent on neurodegeneration is determined is limited. We here address hexanoyl dopamine (HED), one of novel dopamine adducts derived from brain polyunsaturated acid, referring to its in vitro formation, potent toxicity to SH-SY5Y cells, and application to assess the neuroprotective effect of antioxidative food factors. Dopamine is a neurotransmitter, and its deficiency is a characterized feature in Parkinson's disease (PD); thus, HED provides a new insight into the understanding of dopamine biology and pathophysiology of PD and a novel biomarker for the assessment of neuroprotective therapies. We have established an analytical system for the detection of HED and its toxicity to the neuroblstoma cell line, SH-SY5Y cells. Here, we discuss the characteristics of the system and its applications to investigate the neuroprotective effect of several antioxidants that originate from food.

摘要

在理解神经退行性病变方面取得的进展为神经保护疗法的开发创造了新机会，如抗氧化食物因子、生活方式改变和药物。然而，用于确定药物对神经退行性变影响的生物标志物有限。我们在此探讨己酰多巴胺（HED），一种源自脑多不饱和酸的新型多巴胺加合物，涉及其体外形成、对SH-SY5Y细胞的强毒性以及用于评估抗氧化食物因子的神经保护作用。多巴胺是一种神经递质，其缺乏是帕金森病（PD）的一个特征；因此，HED为理解多巴胺生物学和PD的病理生理学提供了新视角，并为评估神经保护疗法提供了一种新型生物标志物。我们建立了一种用于检测HED及其对神经母细胞瘤细胞系SH-SY5Y细胞毒性的分析系统。在此，我们讨论该系统的特性及其在研究几种源自食物的抗氧化剂的神经保护作用方面的应用。