利用乙醛脱氢酶对头颈部鳞状细胞癌肿瘤干细胞进行单一标志物鉴定。
Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase.
机构信息
Department of Molecular and Cell Biology, University of Wisconsin, Madison, Wisconsin, USA.
出版信息
Head Neck. 2010 Sep;32(9):1195-201. doi: 10.1002/hed.21315.
Abstract
BACKGROUND
In accord with the cancer stem cell (CSC) theory, only a small subset of cancer cells are capable of forming tumors. We previously reported that CD44 isolates tumorigenic cells from head and neck squamous cell cancer (HNSCC). Recent studies indicate that aldehyde dehydrogenase (ALDH) activity may represent a more specific marker of CSCs.
METHODS
Six primary HNSCCs were collected. Cells with high and low ALDH activity (ALDH(high)/ALDH(low)) were isolated. ALDH(high) and ALDH(low) populations were implanted into NOD/SCID mice and monitored for tumor development.
RESULTS
ALDH(high) cells represented a small percentage of the tumor cells (1% to 7.8%). ALDH(high) cells formed tumors from as few as 500 cells in 24/45 implantations, whereas only 3/37 implantations of ALDH(low) cells formed tumors.
CONCLUSIONS
ALDH(high) cells comprise a subpopulation cells in HNSCCs that are tumorigenic and capable of producing tumors at very low numbers. This finding indicates that ALDH activity on its own is a highly selective marker for CSCs in HNSCC.
摘要
背景
根据癌症干细胞（CSC）理论，只有一小部分癌细胞能够形成肿瘤。我们之前的报告表明，CD44 可以从头颈部鳞状细胞癌（HNSCC）中分离出肿瘤发生细胞。最近的研究表明，醛脱氢酶（ALDH）活性可能是 CSCs 的更特异性标志物。
方法
收集了 6 个原发性 HNSCC。分离出高 ALDH 活性（ALDH(high)/ALDH(low)）和低 ALDH 活性的细胞。将 ALDH(high)和 ALDH(low)群体植入 NOD/SCID 小鼠中，并监测肿瘤的发展。
结果
ALDH(high)细胞仅占肿瘤细胞的一小部分（1%到 7.8%）。ALDH(high)细胞仅用 500 个细胞就可以在 24/45 次植入中形成肿瘤，而 ALDH(low)细胞仅在 3/37 次植入中形成肿瘤。
结论
ALDH(high)细胞构成了 HNSCC 中具有肿瘤发生能力且能够以极低数量产生肿瘤的细胞亚群。这一发现表明，ALDH 活性本身是 HNSCC 中 CSCs 的高度特异性标志物。
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