Cho Kwang-Jae, Park Eun-Ji, Kim Min-Sik, Joo Young-Hoon
Department of Otolaryngology-Head & Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Department of Otolaryngology-Head & Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Auris Nasus Larynx. 2018 Jun;45(3):566-573. doi: 10.1016/j.anl.2017.07.011. Epub 2017 Aug 24.
The aim of this study was to evaluate the impact of CSC on insensitivity to radiotherapy in HNSCC.
A radioresistant cell line, FaDu-R, was established using fractionated ionizing radiation. Cells with high and low CD44/ALDH activity were isolated.
FaDu-R cells demonstrated significantly increased cell viability after radiation exposure compared with parental cells. CD44/ALDH FaDu-R cells demonstrated significantly faster wound closure (p<0.05) and more efficient invasion (p<0.05) compared to the CD44/ALDH FaDu cells or the CD44/ALDH FaDu-R cells. There was a significant difference in tumor volume between the CD44/ALDH FaDu-R cells and the CD44/ALDH FaDu cells (p<0.05) as well as the CD44/ALDH FaDu-R cells (p<0.05).
Cancer stem cells (CSC) were associated with invasion and tumorigenesis in a radioresistant head and neck squamous cell carcinoma (HNSCC) cell line. This concept might help to improve the understanding of these mechanisms and to develop drugs that can overcome radioresistance during radiotherapy.
本研究旨在评估癌症干细胞（CSC）对头颈部鳞状细胞癌（HNSCC）放疗抵抗的影响。
使用分次电离辐射建立了放射抗性细胞系FaDu-R。分离出具有高和低CD44/ALDH活性的细胞。
与亲本细胞相比，FaDu-R细胞在辐射暴露后表现出显著增加的细胞活力。与CD44/ALDH FaDu细胞或CD44/ALDH FaDu-R细胞相比，CD44/ALDH FaDu-R细胞表现出显著更快的伤口闭合（p<0.05）和更有效的侵袭（p<0.05）。CD44/ALDH FaDu-R细胞与CD44/ALDH FaDu细胞之间的肿瘤体积存在显著差异（p<0.05），与CD44/ALDH FaDu细胞相比也存在显著差异（p<0.05）。
癌症干细胞（CSC）与放射抗性头颈部鳞状细胞癌（HNSCC）细胞系中的侵袭和肿瘤发生相关。这一概念可能有助于增进对这些机制的理解，并开发出能够在放疗期间克服放射抗性的药物。
