Wanchu A, Bhatnagar A, Talreja J, Sapra S, Suryanarayana B S, Suresh P
Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Chest Dis Allied Sci. 2009 Oct-Dec;51(4):207-11.
Tuberculosis (TB) occurs in more than 50% of human immunodeficiency virus (HIV) infected Indian patients. This study was carried out to determine the immunophenotypic and intracellular cytokine profile of patients with HIV-TB co-infection.
Fifteen patients with HIV-TB co-infection and 15 each with TB alone and healthy individuals were studied. Immunophenotypic analysis and intracellular cytokines were measured using appropriate antibodies on a flowcytometer.
Percentage of CD3+ did not differ significantly in the three groups. The ratio of CD4+ : CD8+ was reversed among patients with TB and HIV-TB. CD19+ and CD25+ were present on fewer cells of healthy individuals but this was not statistically significant. Significantly higher percentage of cells of patients with TB and HIV-TB were CD69 positive. Interferon-gamma (INF-gamma) and tumour necrosis factor-alpha (TNF-alpha) levels are significantly reduced in the CD4+ cells of patients with HIV-TB when compared with those with TB and healthy individuals. In CD8+ cells of patients with HIV-TB, levels of TNF-alpha are higher when compared with the other two groups. Interleukin-2 (IL-2) producing cells were not significantly different in any of the above subsets. Monocytes in individuals with HIV-TB had significantly higher interleukin-6 (IL-6) and TNF-alpha.
T-helper cells among patients with HIV-TB have significantly lower cytokine production. T-suppressor cells and monocytes produce more TNF-alpha. These findings may be significant in view of recent attempts to treat HIV-TB coinfected patients with anti-TNF therapy.
在超过50%的感染人类免疫缺陷病毒（HIV）的印度患者中会发生结核病（TB）。本研究旨在确定HIV-TB合并感染患者的免疫表型和细胞内细胞因子谱。
对15例HIV-TB合并感染患者、15例单纯TB患者和15例健康个体进行了研究。使用适当的抗体在流式细胞仪上进行免疫表型分析和细胞内细胞因子检测。
三组中CD3+的百分比无显著差异。TB患者和HIV-TB患者中CD4+与CD8+的比例相反。健康个体中CD19+和CD25+存在于较少的细胞上，但这无统计学意义。TB患者和HIV-TB患者中CD69阳性细胞的百分比显著更高。与TB患者和健康个体相比，HIV-TB患者CD4+细胞中的干扰素-γ（INF-γ）和肿瘤坏死因子-α（TNF-α）水平显著降低。在HIV-TB患者的CD8+细胞中，TNF-α水平高于其他两组。上述任何亚组中产生白细胞介素-2（IL-2）的细胞无显著差异。HIV-TB个体中的单核细胞具有显著更高的白细胞介素-6（IL-6）和TNF-α。
HIV-TB患者中的辅助性T细胞产生细胞因子的能力显著降低。抑制性T细胞和单核细胞产生更多的TNF-α。鉴于最近尝试用抗TNF疗法治疗HIV-TB合并感染患者，这些发现可能具有重要意义。
