γ-干扰素和脂多糖对土拉弗朗西斯菌LVS在小鼠巨噬细胞样细胞系J774中生长的影响
The effect of interferon-gamma and lipopolysaccharide on the growth of Francisella tularensis LVS in murine macrophage-like cell line J774.
作者信息
Holická Monika, Novosad Jakub, Loudová Martina, Kudlová Manuela, Krejsek Jan
机构信息
Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Králové, Czech Republic.
出版信息
Acta Medica (Hradec Kralove). 2009;52(3):101-6. doi: 10.14712/18059694.2016.113.
BACKGROUND
Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells.
AIMS
To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide.
RESULTS
Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection.
CONCLUSIONS
Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis.
背景
土拉弗朗西斯菌是人类兔热病的病原体，具有在人体细胞内增殖的能力。
目的
评估重组干扰素γ和大肠杆菌衍生的脂多糖对巨噬细胞样细胞系J774中土拉弗朗西斯菌LVS菌株生长潜力的影响。
结果
单独用干扰素-γ或脂多糖刺激J774细胞，尤其是在感染土拉弗朗西斯菌之前联合刺激，均显示出保护作用。抑制正在进行的土拉弗朗西斯菌感染需要更高浓度的刺激剂。
结论
干扰素-γ与脂多糖联合刺激J774细胞系可抑制土拉弗朗西斯菌的细胞内生长。
Zotero 插件