Role of neuronal Ca2+-sensor proteins in Golgi-cell-surface membrane traffic.

The regulated local synthesis of PtdIns4P and PtdIns(4,5)P(2) is crucial for TGN (trans-Golgi network)-plasma membrane trafficking. The activity of PI4Kbeta (phosphoinositide 4-kinase IIIbeta) at the Golgi membrane is a first mandatory step in this process. In addition to PI4Kbeta activity, elevated Ca(2+) levels are also needed for the exit of vesicles from the TGN. The reason for this Ca(2+) requirement is at present unclear. In the present review, we discuss the role of neuronal Ca(2+)-sensor proteins in the regulation of PI4Kbeta and suggest that this regulation might impose a need for elevated Ca(2+) levels for a late step of vesicle assembly.