Griffiths G
EMBL, Department of Cell Biology, Postfach 102209, Heidelberg D-69012, Germany.
Traffic. 2000 Sep;1(9):738-45. doi: 10.1034/j.1600-0854.2000.010906.x.
The new millennium coincides within 1 year of Camillo Golgi's centennial celebrations. It is quite remarkable that the structure and formation of this organelle is as controversial today as was its mere existence from Golgi's time to the 1950s, when EM approaches were introduced. Since the late 1950s, two opposing models of Golgi structure and function have split the Golgi scientific community, namely vesicular transport versus organelle maturation. Although a few years ago Golgi maturation seemed to be 'out for the count', it has recently seen an almost messianic revival. In this review, I argue that this large-scale desertion from the vesicle transport model to the maturation camp is premature. I propose an alternative, dynamic steady-state model, in which transient tubular connections function in parallel to vesicular transport and that the biosynthetic pathway is made up of three major distinct compartments: the ER, the Golgi and the TGN.
新千年与卡米洛·高尔基诞辰一百周年庆典在同一年。令人瞩目的是，这个细胞器的结构和形成在今天仍存在争议，就如同从高尔基时代到20世纪50年代引入电子显微镜技术之前其仅仅存在这一事实一样备受争议。自20世纪50年代末以来，关于高尔基结构和功能的两种对立模型将高尔基科学界一分为二，即囊泡运输模型与细胞器成熟模型。尽管几年前高尔基成熟模型似乎已“出局”，但最近它却迎来了近乎救世主般的复兴。在这篇综述中，我认为从囊泡运输模型大规模转向成熟模型的做法为时过早。我提出了一种替代性的动态稳态模型，其中瞬时管状连接与囊泡运输并行发挥作用，且生物合成途径由三个主要不同的区室组成：内质网、高尔基体和反式高尔基体网络。
