Department of Gastrosurgical Research and Education, Institute of Clinical Sciences, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
BMC Gastroenterol. 2010 Jan 14;10:3. doi: 10.1186/1471-230X-10-3.
The role of the renin-angiotensin system in gastric physiology and disease has as yet been sparsely explored. The first aim of the study was to investigate the baseline presence and location of angiotensin II receptors (AT1R and AT2R) in the stomach of the Mongolian gerbil. A second aim was to elucidate whether the presence of H. pylori infection is associated with changes in the expression of these receptors.
H. pylori-negative and H. pylori-infected (strain SS1 or TN2GF4) male Mongolian gerbils were investigated. The stomachs were examined at six or 12 months after inoculation by the use of immunohistochemistry, western blot and microscopic morphometry.
AT1R and AT2R were located in a variety of cells in the gerbil gastric wall, including a subpopulation of endocrine cells in the antral mucosa and inflammatory cells infiltrating H. pylori-infected stomachs. Gerbils infected with the SS1 strain showed a significantly increased antral AT1R protein expression and an increased number of infiltrating polymorphonuclear leucocytes (PMNs) at 12 months. The AT1R protein expression correlated with the number of PMNs and the antral expression of myeloperoxidase.
Angiotensin II receptors are present in a variety of cells in the gastric wall of the Mongolian gerbil. The results indicate an influence dependent on the H. pylori strain on the gastric AT1R expression and a relationship between gastric AT1R expression and mucosal PMNs infiltration.
肾素-血管紧张素系统在胃生理和疾病中的作用尚未得到充分探索。本研究的首要目的是研究胃动素受体（AT1R 和 AT2R）在蒙古沙鼠胃中的基础存在和位置。第二个目的是阐明 H. pylori 感染的存在是否与这些受体表达的变化有关。
研究了 H. pylori 阴性和 H. pylori 感染（菌株 SS1 或 TN2GF4）的雄性蒙古沙鼠。在接种后 6 或 12 个月，通过免疫组织化学、western blot 和显微镜形态计量学检查胃。
AT1R 和 AT2R 存在于蒙古沙鼠胃壁的多种细胞中，包括胃窦黏膜中的内分泌细胞亚群和侵袭 H. pylori 感染胃的炎症细胞。感染 SS1 株的沙鼠在 12 个月时表现出明显增加的胃窦 AT1R 蛋白表达和增加的浸润多形核白细胞（PMN）数量。AT1R 蛋白表达与 PMN 数量和胃窦髓过氧化物酶的表达相关。
血管紧张素 II 受体存在于蒙古沙鼠胃壁的多种细胞中。结果表明，H. pylori 菌株对胃 AT1R 表达有影响，胃 AT1R 表达与黏膜 PMN 浸润有关。
