Elfvin Anders, Bölin Ingrid, Von Bothmer Charlotte, Stolte Manfred, Watanabe Hidenobu, Fändriks Lars, Vieth Michael
Department of Gastrosurgical Research, Sahlgrenska Academy, Göteborg University, Sahlgrensky University Hospital. Box 75038, SE-400 36 Gothenburg, Sweden.
Scand J Gastroenterol. 2005 Nov;40(11):1313-20. doi: 10.1080/00365520510023611.
The Mongolian gerbil is considered as the model of choice when studying adenocarcinoma related to Helicobacter pylori infection. The purpose of this study was to compare two different H. pylori strains and elucidate whether adenocarcinomas developed in gerbils.
Male gerbils were separated into three groups: one control and two groups infected with two different strains of H. pylori, TN2GF4 and SS1. At 3, 6, 12 or 18 months after inoculation 5 animals from each group were sacrificed. The stomach was used for culture, and for histology.
Inflammation was seen after 3 months in all the infected animals. In the controls no pathology was found at any time. Intestinal metaplasia was found in both the infected groups. Glands buried in the submucusal layer, changes that might be misinterpreted as adenocarcinoma, were found in 10% of the SS1 and in 65% of the TN2GF4 animals. Adenocarcinoma was not found in any of the gerbils.
All studies claiming to have found H. pylori-induced adenocarcinomas in gerbils describe atypical glands penetrating into the muscularis propria and interpret these as invasive growths due to cancer. An alternative interpretation is that the deranged glandular structures grow in and below the submucosa. It is suggested that atypical glands in the muscularis layer are not enough as a diagnostic criterion for gastric adenocarcinoma. It is concluded that adenocarcinoma has not yet been shown convincingly to develop in Mongolian gerbils infected with H. pylori. Nevertheless, it is a model well suited for studying gastritis, gastric ulcer and premalignant changes such as metaplasia.
蒙古沙鼠被认为是研究幽门螺杆菌感染相关腺癌的首选模型。本研究的目的是比较两种不同的幽门螺杆菌菌株，并阐明沙鼠是否会发生腺癌。
将雄性沙鼠分为三组：一组为对照组，两组分别感染两种不同的幽门螺杆菌菌株TN2GF4和SS1。接种后3、6、12或18个月，每组处死5只动物。取胃进行培养和组织学检查。
所有感染动物在3个月后均出现炎症。对照组在任何时候均未发现病理改变。两个感染组均发现肠化生。在SS1组10%的动物和TN2GF4组65%的动物中发现了埋于黏膜下层的腺体，这些改变可能被误诊为腺癌。所有沙鼠均未发现腺癌。
所有声称在沙鼠中发现幽门螺杆菌诱导腺癌的研究均描述了穿透固有肌层的非典型腺体，并将其解释为癌症的浸润性生长。另一种解释是，紊乱的腺结构在黏膜下层及其下方生长。提示肌层中的非典型腺体不足以作为胃腺癌的诊断标准。结论是，尚未有令人信服的证据表明感染幽门螺杆菌的蒙古沙鼠会发生腺癌。然而，它是一个非常适合研究胃炎、胃溃疡和化生等癌前病变的模型。
