抗风湿药物对DBA/1J小鼠Ⅱ型胶原诱导性关节炎的病理学评估

Pathological evaluation of anti-rheumatic drugs on type II collagen-induced arthritis in DBA/1J mouse.

作者信息

Imaizumi K, Hinoue H, Ueno M, Takata I, Sato T, Minato Y, Takeshita M, Okaniwa A

机构信息

Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.

出版信息

Jikken Dobutsu. 1991 Jan;40(1):95-9. doi: 10.1538/expanim1978.40.1_95.

DOI:10.1538/expanim1978.40.1_95

PMID:2007440

Abstract

The effects of anti-rheumatic drugs (lobenzarit (CCA); 10 and 50mg/kg, cyclophosphamide (CP); 5 mg/kg and dexamethasone (DM); 0.25mg/kg) were evaluated immunologically and histopathologically on DBA/1J mice that develop polyarthritis after immunization by the intradermal injection of type II collagen. Serum anti-type II collagen IgG levels in the groups treated with CP and DM were significantly suppressed to 1/2 and 1/10 as compared to those of the positive control group, respectively. Those of both groups treated with CCA were not different from those of the positive control group. Histopathological examination revealed that treatment with CP and DM markedly reduced or suppressed inflammatory changes and resulted in low incidence of arthritis. From the standpoint mentioned above, treatment with anti-rheumatic drugs suppressed the development of arthritis in this model, and we could confirm that this model was useful for evaluation of the effect of anti-rheumatic drugs.

摘要

通过对经皮内注射II型胶原蛋白免疫后发生多关节炎的DBA/1J小鼠进行免疫和组织病理学评估，研究了抗风湿药物（氯苯扎利（CCA）；10和50mg/kg、环磷酰胺（CP）；5mg/kg和地塞米松（DM）；0.25mg/kg）的效果。与阳性对照组相比，CP和DM治疗组的血清抗II型胶原蛋白IgG水平分别显著抑制至1/2和1/10。CCA治疗的两组与阳性对照组无差异。组织病理学检查显示，CP和DM治疗显著减少或抑制了炎症变化，且关节炎发病率较低。从上述观点来看，抗风湿药物治疗抑制了该模型中关节炎的发展，我们可以确认该模型对于评估抗风湿药物的效果是有用的。