Phadke K, Fouts R L, Parrish J E, Butler L D
Immunopharmacology. 1985 Aug;10(1):51-60. doi: 10.1016/0162-3109(85)90059-1.
A battery of drugs which are commonly used as therapeutic agents for arthritis was tested for effects on the inflammatory and immunological responses of DBA/1J mice, after immunization with type II collagen. All the drugs were tested at more than one dosage. The mice were protected from the development of arthritis by treatment with paramethasone (0.25 mg/kg/day) or cyclophosphamide (5 mg/kg/day). The nonsteroidal anti-inflammatory drugs used in these studies, viz. aspirin (200 mg/kg/day), benoxaprofen (100 mg/kg/day) and naproxen (200 mg/kg/day), had no significant effect on the joint involvement, although naproxen and benoxaprofen at these high doses caused some reduction of immune responses of mice to collagen. Chloroquine (100 mg/kg/day), levamisol (50 mg/kg/day) and gold chlorophosphene (5 mg/kg/day) had no effect on the inflammatory or humoral response, while treatment with D-penicillamine (100 mg/kg/day) led to an early onset of arthritis in mice. These data suggest that the type II collagen-induced mouse arthritis model may not be highly suitable for detection of the traditional nonsteroidal anti-inflammatory class of drugs or the anti-rheumatic drugs, although the possibility remains that some new and novel immunosuppressive agents may be detected with this model.
用II型胶原免疫DBA/1J小鼠后，对一组常用于治疗关节炎的药物进行了测试，观察其对小鼠炎症和免疫反应的影响。所有药物均采用多种剂量进行测试。用对氟米松（0.25毫克/千克/天）或环磷酰胺（5毫克/千克/天）治疗可保护小鼠不发生关节炎。这些研究中使用的非甾体抗炎药，即阿司匹林（200毫克/千克/天）、苯恶洛芬（100毫克/千克/天）和萘普生（200毫克/千克/天），对关节受累情况无显著影响，尽管萘普生和苯恶洛芬在这些高剂量下会使小鼠对胶原的免疫反应有所降低。氯喹（100毫克/千克/天）、左旋咪唑（50毫克/千克/天）和氯磷金（5毫克/千克/天）对炎症或体液反应无影响，而用D-青霉胺（100毫克/千克/天）治疗会导致小鼠关节炎提前发作。这些数据表明，II型胶原诱导的小鼠关节炎模型可能不太适合检测传统的非甾体抗炎药或抗风湿药，尽管仍有可能通过该模型检测到一些新型的免疫抑制剂。
