Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China.
Fish Shellfish Immunol. 2010 Apr;28(4):727-33. doi: 10.1016/j.fsi.2010.01.002. Epub 2010 Jan 14.
In this study we found that a blue shrimp (Litopenaeus stylirostris) lysozyme gene (Lslzm) was up-regulated in WSSV-infected shrimp, suggesting that lysozyme is involved in the innate response of shrimp to this virus. Shrimp were intramuscularly injected with Lslzm protein to identify how this recombinant protein protects L. stylirostris from WSSV infection and to determine how this protein influences nonspecific cellular and humoral defense mechanisms. Higher survival rates and a lower viral load (compared with controls) were reported for shrimps that were first injected with the Lslzm protein and then infected with WSSV. In addition, the Lslzm expression level and the immunological parameters (including THC, phagocytic activity, respiratory burst activity, phenoloxidase activity and lysozyme activity) were all significantly higher in the WSSV-infected shrimp treated with the Lslzm protein, compared with the controls. These results indicate that lysozyme is effective at blocking WSSV infection in L. stylirostris and that lysozyme modulates the cellular and humoral defense mechanisms after they are suppressed by the WSSV virus.
在这项研究中，我们发现凡纳滨对虾溶菌酶基因（Lslzm）在 WSSV 感染的虾中被上调，表明溶菌酶参与了虾对这种病毒的先天反应。我们通过肌肉注射 Lslzm 蛋白来鉴定这种重组蛋白如何保护凡纳滨对虾免受 WSSV 感染，并确定这种蛋白如何影响非特异性细胞和体液防御机制。与对照组相比，首先注射 Lslzm 蛋白然后感染 WSSV 的虾的存活率更高，病毒载量更低。此外，与对照组相比，用 Lslzm 蛋白处理的感染 WSSV 的虾的 Lslzm 表达水平和免疫参数（包括 THC、吞噬活性、呼吸爆发活性、酚氧化酶活性和溶菌酶活性）均显著升高。这些结果表明，溶菌酶能有效阻止 WSSV 感染凡纳滨对虾，并且溶菌酶能在 WSSV 病毒抑制后调节细胞和体液防御机制。
