State Key Laboratory of Marine Pollution and Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong.
Sci Total Environ. 2010 Feb 15;408(6):1458-65. doi: 10.1016/j.scitotenv.2009.12.044. Epub 2010 Jan 13.
Lysosomal integrity in mussels is widely used as a biomarker in coastal environments to demonstrate exposure to trace organic pollutants. However, few studies have determined the long-term influences of seasonal variations on the bioaccumulation of trace organics and subsequently altered response of lysosomal integrity in mussels. This study aimed to test three null hypotheses that (1) bioaccumulations of total polycyclic aromatic hydrocarbon (SigmaPAH) and (2) total polychlorinated biphenyl (SigmaPCB), and (3) lysosomal integrity as indicated by Neutral Red retention time (NRRT) in haemocytes, in the green-lipped mussel Perna viridis were not seasonally dependent. The tissue concentrations of SigmaPAH and SigmaPCB and haemocytic NRRT were determined in P. viridis in a metropolitan harbour, subtropical Hong Kong during the wet and dry seasons from 2004 to 2007. Additional information on temperature, salinity, dissolved oxygen and total ammonia nitrogen in seawater, and sediment levels of SigmaPAH and SigmaPCB, were extracted from published data and re-analyzed. Our results accepted all null hypotheses, based on the minimal seasonal influences of seawater temperature and salinity on all studied parameters, in which no significant differences between the wet and dry seasons were detected. The seasonal effect was likely outweighed by the greatly improved water quality and pollution abatement noted inside the harbour, with a gradual shift in mussel PAHs from a pyrolytic origin to a petrogenic origin. Spatially, the site east of the harbour was relatively unpolluted. The single use of NRRT in P. viridis explained 25% of the total variation of the integrated pollution patterns in seawater, sediments and mussels. The present study suggested that the dynamic change of trace organics could be reflected by the response on lysosomal integrity in P. viridis, which was recommended as a routine screening biomarker in monitoring of harbour water quality across seasons.
贻贝中的溶酶体完整性被广泛用作沿海环境中痕量有机污染物暴露的生物标志物。然而,很少有研究确定季节性变化对痕量有机物生物累积的长期影响,以及随后对贻贝溶酶体完整性的反应的改变。本研究旨在检验三个零假设,即:(1) 总多环芳烃 (SigmaPAH) 和 (2) 总多氯联苯 (SigmaPCB) 的生物累积,以及 (3) 血细胞中性红保留时间 (NRRT) 指示的溶酶体完整性,在亚热带香港的都会港中的绿唇贻贝 Perna viridis 中不受季节影响。在 2004 年至 2007 年的湿季和干季,从已发表的数据中提取了有关温度、盐度、溶解氧和总氨氮以及 SigmaPAH 和 SigmaPCB 沉积物水平的额外信息,并进行了重新分析。我们的结果接受了所有的零假设,这是基于海水温度和盐度对所有研究参数的最小季节性影响,在湿季和干季之间没有检测到显著差异。由于港湾内水质的改善和污染的减少,季节效应可能被大大超过,贻贝中的多环芳烃逐渐从热解来源转变为生源来源。在空间上,港湾东部的地点相对无污染。NRRT 在 P. viridis 中的单一使用解释了海水、沉积物和贻贝中综合污染模式总变异的 25%。本研究表明,痕量有机物的动态变化可以通过贻贝溶酶体完整性的反应来反映,建议将其作为监测港湾水质的常规筛选生物标志物。