Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Campus Montilivi, Girona, Spain.
Antonie Van Leeuwenhoek. 2011 Feb;99(2):403-8. doi: 10.1007/s10482-010-9412-x. Epub 2010 Jan 14.
Culture conditions for the maintenance of previously uncultured members of the Archaea thriving in anoxic water layers of stratified freshwater lakes are described. The proposed enrichment conditions, based on the use of defined medium composition and the maintenance of anoxia, have been proven effective for the maintenance of the archaeal community with virtually no changes over time for periods up to 6 months as revealed by a PCR-DGGE analysis. Phylotypes belonging to groups poorly represented in culture collections such as the Deep-Sea Hydrothermal Vent Euryarchaeota (DHVE) and the Miscellaneous Crenarchaeotic Group (MCG) were maintained and selectively enriched when compared to the correspondent indigenous planktonic archaeal community.
介绍了用于维持在分层淡水湖中缺氧水层中茁壮成长的以前无法培养的古菌成员的培养条件。所提出的基于使用限定培养基组成和维持缺氧的富集条件已被证明在长达 6 个月的时间内对古菌群落的维持是有效的，PCR-DGGE 分析显示几乎没有随时间发生变化。与相应的土著浮游古菌群落相比，当与深海水热喷口广古菌（DHVE）和杂类 Crenarchaeotic 组（MCG）等在培养物中代表性较差的类群的系统发育型时，它们被维持和选择性富集。
