Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy.
Ann Biomed Eng. 2010 Mar;38(3):640-8. doi: 10.1007/s10439-009-9898-4.
When isolated from the iliac crest human mesenchymal stem cells (hMSC) differentiate into osteoblast-like cells with appropriate stimulation in culture. This in vitro study tested the hypothesis that Biostite and hydroxyapatite (HA) affect proliferation and differentiation of hMSC into osteoblastic cells. Cell proliferation was determined by measuring 3H-thymidine incorporation into DNA and typical markers of osteoblastic phenotype were determined by RT-PCR assay. No differences emerged in cell proliferation cultures with Biostite or hydroxyapatite (HA), but gene expression analysis revealed higher expression of collagen,alkaline phosphatase (ALP), osteopontin and bone sialoprotein (BSP) in the presence of Biostite. TGFb2 production, as assessed by an Elisa kit, and Runx2 expression by RT-PCR, were greater in Biostite cultures, suggesting Biostite provides a better environment for hMSC differentiation into osteoblasts and is, potentially, a more promising bone-filling material than HA.
从髂嵴分离的人骨髓间充质干细胞（hMSC）在适当的刺激下可在体外分化为成骨样细胞。本体外研究旨在验证 Biostite 和羟基磷灰石（HA）是否会影响 hMSC 向成骨细胞分化和增殖这一假说。通过测量 3H-胸腺嘧啶核苷掺入 DNA 来确定细胞增殖，通过 RT-PCR 分析测定成骨细胞表型的典型标志物。Biostite 或羟基磷灰石（HA）对细胞增殖培养无差异，但基因表达分析显示 Biostite 存在时胶原、碱性磷酸酶（ALP）、骨桥蛋白和骨涎蛋白（BSP）的表达更高。通过 Elisa 试剂盒评估 TGFb2 的产生，通过 RT-PCR 评估 Runx2 的表达，Biostite 培养物中的表达更高，这表明 Biostite 为 hMSC 向成骨细胞分化提供了更好的环境，并且可能是比 HA 更有前途的骨填充材料。
