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灌注条件下基于壳聚糖的支架中羟基磷灰石含量对人间充质干细胞分化的调控

Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions.

作者信息

Rogina Anamarija, Antunović Maja, Pribolšan Lidija, Caput Mihalić Katarina, Vukasović Andreja, Ivković Alan, Marijanović Inga, Gallego Ferrer Gloria, Ivanković Marica, Ivanković Hrvoje

机构信息

Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10001 Zagreb, Croatia.

Faculty of Science, University of Zagreb, Horvatovac102a, 10001 Zagreb, Croatia.

出版信息

Polymers (Basel). 2017 Aug 23;9(9):387. doi: 10.3390/polym9090387.

DOI:10.3390/polym9090387
PMID:30965692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6418638/
Abstract

The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold's components with positive influence on osteoinduction and differentiation of human mesenchymal stem cells, in terms of their higher fraction within the graft. Nevertheless, the creation of unlimited nutrients diffusion through newly formed grafts is of great importance. The media flow accomplished by perfusion forces can provide physicochemical, and also, biomechanical stimuli for three-dimensional bone-construct growth. In the present study, the influence of a different scaffold's composition on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning was investigated. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes' expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10⁻30% within chitosan scaffold could be preferable for bone-construct development.

摘要

对硬组织替代物的广泛需求极大地推动了用于治疗性修复的合适同种异体移植物的开发。不同的磷酸钙相已被用作支架成分，因其在移植物中所占比例较高，对人间充质干细胞的骨诱导和分化具有积极影响。然而，通过新形成的移植物实现无限的营养物质扩散非常重要。灌注力实现的培养基流动可为三维骨构建物的生长提供物理化学和生物力学刺激。在本研究中，研究了在灌注条件下于U-CUP生物反应器中不同支架组成对人间充质干细胞（hMSCs）分化的影响。对培养的骨组织进行组织学和免疫组织化学分析，以及对成骨基因表达的评估表明，壳聚糖支架中10%-30%范围内原位形成的羟基磷灰石较低比例可能更有利于骨构建物的发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/07a007106730/polymers-09-00387-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/a1b0a1f65258/polymers-09-00387-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/e21e16aa117f/polymers-09-00387-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/f23956c07ad2/polymers-09-00387-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/b458c364629e/polymers-09-00387-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/9638044f20aa/polymers-09-00387-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/f541e4295a8c/polymers-09-00387-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/07a007106730/polymers-09-00387-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/a1b0a1f65258/polymers-09-00387-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/e21e16aa117f/polymers-09-00387-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/f23956c07ad2/polymers-09-00387-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/b458c364629e/polymers-09-00387-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/9638044f20aa/polymers-09-00387-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/f541e4295a8c/polymers-09-00387-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0342/6418638/07a007106730/polymers-09-00387-g007.jpg

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