Trombelli L, Penolazzi L, Torreggiani E, Farina R, Lambertini E, Vecchiatini R, Piva R
Medico-Surgical Disciplines of Communication and Behaviour Department, Research Centre for the Study of Periodontal Diseases, University of Ferrara, Ferrara, Italy.
Minerva Stomatol. 2010 Mar;59(3):103-15.
The present study evaluated human primary osteoblasts and two different osteoblast-like cell lines behaviour when cultured in presence of different hydroxyapatite-based (HA) biomaterials (SINTlife-FIN-CERAMICA S.p.a., Faenza, Italy; Bio-Oss, Geistlich Biomaterials, Woulhusen, Switzerland; Biostite-GABA Vebas, San Giuliano Milanese, MI, Italy), focusing attention on the effect of HA/Biostite in terms of modulation of osteoblastic differentiation. Analysis were about adhesion, proliferation and mineralization activity. Runt-related transcription factor 2 (Runx2), Estrogen Receptor alpha (ERalfa) expression and alkaline phosphatase activity (ALP) were measured as osteoblastic differentiation markers. Determination of viable cells was done with MTT colorimetric assay. Scanning electron microscopy (SEM) analysis was performed on biomaterial-treated cells. All hydroxyapatite-based biomaterials didn't affect cells morphology and viability, whereas only presence of HA/Biostite improved cells adhesion, growth and differentiation. Adhesion and spreading of the primary cells on HA/Biostite were the same showed by two different osteoblast-like cell lines. These results have important implications for both tissue-engineered bone grafts and enhancement of HA implants performance, to develop new teeth's supporting structure therapies and replacement.
本研究评估了人原代成骨细胞和两种不同的成骨样细胞系在不同羟基磷灰石基（HA）生物材料（SINTlife - FIN - CERAMICA S.p.a.，意大利法恩扎；Bio - Oss，Geistlich Biomaterials，瑞士伍尔胡森；Biostite - GABA Vebas，意大利米兰圣朱利亚诺）存在下的行为，重点关注HA/Biostite在调节成骨细胞分化方面的作用。分析内容包括黏附、增殖和矿化活性。测量了与成骨细胞分化标志物相关的 runt 相关转录因子 2（Runx2）、雌激素受体α（ERalfa）的表达以及碱性磷酸酶活性（ALP）。用MTT比色法测定活细胞。对生物材料处理过的细胞进行扫描电子显微镜（SEM）分析。所有羟基磷灰石基生物材料均不影响细胞形态和活力，而只有HA/Biostite的存在改善了细胞的黏附、生长和分化。原代细胞在HA/Biostite上的黏附和铺展情况与两种不同的成骨样细胞系相同。这些结果对于组织工程骨移植以及提高HA植入物性能、开发新的牙齿支撑结构治疗方法和替代物都具有重要意义。
