SMALL, Nanobio Sensors and MicroActuators LearningLaboratory) Department of Electrical Engineering, University at Buffalo, The State University of New ,Buffalo, NY 14260, USA.
Biomed Microdevices. 2010 Apr;12(2):297-309. doi: 10.1007/s10544-009-9385-6.
We present a 2-layer based microfluidic concentration generator by a hybrid of a serial and a volumetric dilution for dose-response experiments in drug screening. The hybrid dilution method using 2-layer based microfluidic network significantly reduces the total number of cascaded serial dilution stages. The proposed strategy is capable of generating a large number of universal stepwise monotonic concentrations with a wide range of logarithmic and linear scales. We have studied an equivalent electrical circuit to that of the 2-layer based microfluidic network, where the only variable parameter is channel length. We have designed a microfluidic dilution generator simultaneously covering 14 doses with a combination of 4-order logarithmic and 4-point linear concentrations. The design has been verified by a commercial circuit analysis software (e.g., P-Spice) for the electrical circuit analysis and a computational fluid dynamics software (e.g., CFD-ACE+) for the microfluidic circuit analysis. As a real-life application of the proposed dilution generator, we have successfully performed a dose-response experiment using MCF-7 human breast cancer cells. We expect that the proposed dilution method will be useful to study not only high throughput drug screening but also optimization in biology, chemistry, medicine, and material sciences.
我们提出了一种基于两层的微流控浓度发生器，采用串联和体积稀释的混合方法，用于药物筛选中的剂量反应实验。这种使用两层微流控网络的混合稀释方法显著减少了级联串联稀释阶段的总数。所提出的策略能够生成大量具有广泛对数和线性比例的通用阶梯式单调浓度。我们研究了与两层微流控网络等效的电路，其中唯一的可变参数是通道长度。我们设计了一种微流控稀释发生器，同时覆盖了 14 种剂量，组合了 4 阶对数和 4 点线性浓度。该设计已通过商业电路分析软件（例如 P-Spice）进行了电路分析，并通过计算流体动力学软件（例如 CFD-ACE+）进行了微流控电路分析进行了验证。作为所提出的稀释发生器的实际应用，我们已经成功地使用 MCF-7 人乳腺癌细胞进行了剂量反应实验。我们预计，所提出的稀释方法不仅将有助于高通量药物筛选的研究，而且还将有助于生物学、化学、医学和材料科学中的优化。
