Molinaro Ross J, Ritchie James C
Department of Pathology and Laboratory Medicine, Emory University School of Medicine and Emory Healthcare Systems, Atlanta, GA, USA.
Methods Mol Biol. 2010;603:307-15. doi: 10.1007/978-1-60761-459-3_29.
The following chapter describes a method to measure iothalamate in plasma and urine samples using high performance liquid chromatography combined with electrospray positive ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Methanol and water are spiked with the internal standard (IS) iohexol. Iothalamate is isolated from plasma after IS spiked methanol extraction and from urine by IS spiked water addition and quick-spin filtration. The plasma extractions are dried under a stream of nitrogen. The residue is reconstituted in ammonium acetate-formic acid-water. The reconstituted plasma and filtered urine are injected into the HPLC-ESI-MS/MS. Iothalamate and iohexol show similar retention times in plasma and urine. Quantification of iothalamate in the samples is made by multiple reaction monitoring using the hydrogen adduct mass transitions, from a five-point calibration curve.
以下章节描述了一种使用高效液相色谱结合电喷雾正离子串联质谱法（HPLC-ESI-MS/MS）测量血浆和尿液样本中碘他拉酸盐的方法。甲醇和水加入内标（IS）碘海醇。血浆经加入内标的甲醇萃取后分离出碘他拉酸盐，尿液则通过加入内标的水并快速离心过滤进行处理。血浆提取物在氮气流下吹干。残渣用醋酸铵 - 甲酸 - 水复溶。复溶后的血浆和过滤后的尿液注入HPLC-ESI-MS/MS。碘他拉酸盐和碘海醇在血浆和尿液中显示出相似的保留时间。通过五点校准曲线，利用氢加合物质量跃迁的多反应监测对样本中的碘他拉酸盐进行定量。
