Molinaro Ross J
Department of Pathology and Laboratory Medicine, Emory University School of Medicine and Emory Healthcare Systems, Atlanta, GA, USA.
Methods Mol Biol. 2010;603:57-63. doi: 10.1007/978-1-60761-459-3_6.
The following chapter describes a method to measure argatroban in plasma samples using ultra-performance liquid chromatography combined with electrospray positive ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Samples are pre-treated using methanol containing the internal standard (IS) diclofenac. The plasma extracts are dried under a stream of nitrogen. The residue is reconstituted in ammonium acetate-formic acid-water. The reconstituted plasma is injected into the UPLC-ESI-MS/MS. Argatroban and diclofenac show similar retention times in plasma. Quantification of argatroban in the samples is made by multiple reaction monitoring using the hydrogen adduct mass transitions, from a seven-point calibration curve.
以下章节介绍了一种使用超高效液相色谱结合电喷雾正离子串联质谱法（UPLC-ESI-MS/MS）测量血浆样本中阿加曲班的方法。样本使用含有内标（IS）双氯芬酸的甲醇进行预处理。血浆提取物在氮气流下干燥。残留物用乙酸铵-甲酸-水复溶。复溶后的血浆注入UPLC-ESI-MS/MS。阿加曲班和双氯芬酸在血浆中的保留时间相似。通过使用氢加合物质量跃迁的多反应监测，根据七点校准曲线对样本中的阿加曲班进行定量。
