气相色谱-质谱法测定美沙酮和2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）。

Gas chromatography-mass spectrometry method for the determination of methadone and 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP).

作者信息

Snozek Christine L H, Bjergum Matthew W, Langman Loralie J

机构信息

Department of Laboratory Medicine and Pathology, Mayo Clinic, Toxicology and Drug Monitoring Laboratory, Rochester, MN, USA.

出版信息

Methods Mol Biol. 2010;603:351-8. doi: 10.1007/978-1-60761-459-3_33.

DOI:10.1007/978-1-60761-459-3_33

PMID:20077086

Abstract

Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, and wean heroin addicts. Measurement of methadone and its major metabolite EDDP in urine is useful for assessing compliance with addiction rehabilitation and pain management programs. This method quantitatively measures methadone and its metabolite EDDP in urine. Methadone and EDDP are recovered from urine by solid phase extraction at pH 6.0. Analysis is performed by gas chromatography with mass spectrometry detection, using selective ion monitoring.

摘要

美沙酮是一种合成阿片类药物，用于缓解疼痛、治疗阿片类药物戒断症状以及使海洛因成瘾者戒毒。检测尿液中的美沙酮及其主要代谢物EDDP有助于评估对成瘾康复和疼痛管理项目的依从性。本方法可定量检测尿液中的美沙酮及其代谢物EDDP。美沙酮和EDDP在pH 6.0条件下通过固相萃取从尿液中回收。采用选择性离子监测，通过气相色谱-质谱联用进行分析。