采用气相色谱-质谱联用仪（GC-MS）对尿液和血液中的苯环己哌啶（PCP）进行定量分析。

Quantitation of phencyclidine (PCP) in urine and blood using gas chromatography-mass spectrometry (GC-MS).

作者信息

Ferguson Angela M, Garg Uttam

机构信息

Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO, USA.

出版信息

Methods Mol Biol. 2010;603:461-7. doi: 10.1007/978-1-60761-459-3_45.

DOI:10.1007/978-1-60761-459-3_45

PMID:20077098

Abstract

Phencyclidine (PCP) is a cycloalkylamine and is classified as a dissociative anesthetic. In the 1950s, PCP was tested as an intravenous anesthetic but due to its severe side effects, it was withdrawn from the clinical use. Since then PCP has become an illegal street drug making its laboratory analysis forensically essential. PCP can be detected in urine, serum, or plasma by immunoassays and quantified by gas or liquid chromatography mass spectrometry. In the method described here, a deuterated internal standard is added to the sample and the drug is extracted under alkaline conditions. Analysis is conducted using gas chromatography mass spectrometry (GC-MS). Quantitation of PCP is done by comparing the responses of unknown samples to the standards using selected ion monitoring.

摘要

苯环己哌啶（PCP）是一种环烷基胺，被归类为分离麻醉剂。在20世纪50年代，PCP被作为静脉麻醉剂进行测试，但由于其严重的副作用，它被停止临床使用。从那时起，PCP已成为一种非法街头毒品，因此对其进行实验室分析在法医学上至关重要。PCP可以通过免疫测定法在尿液、血清或血浆中检测到，并通过气相或液相色谱质谱法进行定量。在此处描述的方法中，将氘代内标添加到样品中，并在碱性条件下提取药物。使用气相色谱质谱法（GC-MS）进行分析。通过使用选择离子监测比较未知样品与标准品的响应来进行PCP的定量。