采用气相色谱-质谱联用仪（GC-MS）对血液和尿液中的羟考酮进行定量分析。

Quantitation of oxycodone in blood and urine using gas chromatography-mass spectrometry (GC-MS).

作者信息

Goldberger Bruce A, Chronister Chris W, Merves Michele L

机构信息

Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.

出版信息

Methods Mol Biol. 2010;603:453-60. doi: 10.1007/978-1-60761-459-3_44.

DOI:10.1007/978-1-60761-459-3_44

PMID:20077097

Abstract

Oxycodone is a semisynthetic opioid analgesic used in pain management. In the following method, gas chromatography-mass spectrometry (GC-MS) is used to determine the presence and concentration of the drug in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted using mixed-mode solid phase extraction and derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) producing trimethylsilyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.

摘要

羟考酮是一种用于疼痛管理的半合成阿片类镇痛药。在以下方法中，采用气相色谱 - 质谱联用仪（GC - MS）来测定血液和尿液中该药物的存在及浓度。样本用氘代内标进行强化，并构建五点校准曲线。样本采用混合模式固相萃取进行提取，并用N,O - 双（三甲基硅基）三氟乙酰胺（BSTFA）进行衍生化反应，生成三甲基硅基衍生物。然后使用选择离子监测GC - MS对最终提取物进行分析。