Human Pathologies and Oxidative Stress Unit, Higher Institute of Biotechnology of Sfax, Tunisia.
Environ Toxicol. 2011 Jun;26(3):287-91. doi: 10.1002/tox.20555.
Organophosphorus insecticides may induce oxidative stress leading to the generation of free radicals and alteration in the antioxidant system. The aim of this study was to examine the potency of Dimethoate (Dim) to induce oxidative stress response in human erythrocyte in vitro and the role of Vitamins C (Vit C) and E (Vit E) in alleviating the cytotoxic effects. Erythrocytes were divided into three groups. The first group, erythrocytes were incubated for 4 h at 37 °C with different concentrations (0, 20, 40, 60, 80, and 100 mM) of Dim. The second and third groups were preincubated with Vit C or Vit E, respectively, for 30 min and followed by Dim incubation for 4 h at 37 °C. Following in vitro exposure, Dim caused a significant increase in malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) in erythrocytes at different concentrations. Vit E or Vit C pretreated erythrocytes showed a significant protection against the cytotoxic effects inducted by Dim on the studied parameters. In conclusion, antioxidant Vit E and C could protect against Dim-induced oxidative stress by decreasing lipid peroxidation and hyperactivity of SOD and CAT in human erythrocytes.
有机磷杀虫剂可能会诱导氧化应激，导致自由基的产生和抗氧化系统的改变。本研究旨在研究乐果（Dim）在体外诱导人红细胞氧化应激反应的能力，以及维生素 C（Vit C）和维生素 E（Vit E）在减轻细胞毒性作用中的作用。将红细胞分为三组。第一组，红细胞在 37°C 下孵育 4 小时，用不同浓度（0、20、40、60、80 和 100mM）的 Dim 处理。第二组和第三组分别用 Vit C 或 Vit E 预孵育 30 分钟，然后在 37°C 下孵育 4 小时。体外暴露后，Dim 在不同浓度下引起红细胞丙二醛（MDA）水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的显著增加。用 Vit E 或 Vit C 预处理的红细胞对 Dim 诱导的研究参数的细胞毒性作用表现出显著的保护作用。总之，抗氧化剂 Vit E 和 C 可以通过降低脂质过氧化和 SOD 和 CAT 的过度活性来防止 Dim 诱导的人红细胞氧化应激。
