Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, Sfax, Tunisia.
Andrologia. 2012 May;44 Suppl 1:272-9. doi: 10.1111/j.1439-0272.2011.01177.x. Epub 2011 Aug 2.
Organophosphorus compounds are currently among the most frequently used pesticides worldwide, and therefore, the potential for human exposure to man is considerable. Their toxicity results in negative effects on many organs and systems such as the male reproductive system. So, vitamins that can offer spermatozoa protection are of great importance. This study was designed to investigate (i) the possibility of dimethoate, an organophosphate insecticide, to induce oxidative stress response in rat spermatozoa in vitro and its effect on antioxidant defence system and (ii) the role of vitamin C and vitamin E in alleviating the cytotoxic effects of dimethoate Epididymal spermatozoa were incubated for 3 h at 37 °C with different concentrations of dimethoate (50, 100 and 200 μm) without vitamins or pre-incubated with 20 mm of vitamin C or 2 mm of vitamin E. Sperm parameters, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels were performed. Dimethoate caused a significant induction of oxidative damage in spermatozoa at different concentrations as evidenced by increased MDA levels. However, a significant decrease in sperm mobility, viability and activities SOD, CAT and GPx was observed. Vitamins pre-treated spermatozoa showed a significant protection against the cytotoxic effects induced by dimethoate on studied parameters.
有机磷化合物目前是世界范围内使用最广泛的农药之一，因此，人类接触的可能性相当大。它们的毒性会对许多器官和系统产生负面影响，如男性生殖系统。因此，能够为精子提供保护的维生素非常重要。本研究旨在探讨（i）有机磷杀虫剂乐果是否有可能在体外诱导大鼠精子产生氧化应激反应，以及其对抗氧化防御系统的影响；（ii）维生素 C 和维生素 E 在减轻乐果细胞毒性作用中的作用。将附睾精子在 37°C 下与不同浓度的乐果（50、100 和 200 μm）孵育 3 小时，不添加维生素或预先用 20mm 维生素 C 或 2mm 维生素 E 孵育。检测精子参数、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平。结果表明，乐果在不同浓度下显著诱导了精子的氧化损伤，表现为 MDA 水平升高。然而，精子的运动能力、活力以及 SOD、CAT 和 GPx 的活性显著下降。预先用维生素处理的精子对乐果诱导的研究参数的细胞毒性作用表现出显著的保护作用。
