槲皮素对乐果诱导的人外周血淋巴细胞氧化应激的保护作用。
Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes.
机构信息
Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, 3038 Tunisie.
出版信息
Lipids Health Dis. 2011 Aug 23;10:149. doi: 10.1186/1476-511X-10-149.
BACKGROUND
The aim of this study is to investigate the effect of quercetin in alleviating the cytotoxic effects of Dimethoate in human peripheral blood lymphocytes.
METHODS
Lymphocytes were divided into too groups. The first group, lymphocytes were incubated for 4 h at 37°C with different concentrations (0, 40, 60 and 100 mM) of Dimethoate. The second group was preincubated with quercetin for 30 min and followed by Dim incubation for 4 h at 37°C.
RESULTS
Following in vitro incubation, Dimethoate caused a significant increase in malondialdehyde levels, a significant decrease in thiol levels, as well as a significant increase in superoxide dismutase, and catalase activities in lymphocytes at different concentrations. Quercetin pretreated lymphocytes showed a significant protection against the cytotoxic effects inducted by Dimethoate on the studied parameters.
CONCLUSION
In conclusion, antioxidant quercetin could protect against Dimethoate-induced oxidative stress by decreasing lipid peroxidation, protein oxidation and increasing superoxide dismutase and catalase activities in human lymphocytes.
背景
本研究旨在探讨槲皮素对减轻乐果对人外周血淋巴细胞细胞毒性的作用。
方法
将淋巴细胞分为两组。第一组，淋巴细胞在 37°C 下用不同浓度（0、40、60 和 100mM）的乐果孵育 4 小时。第二组用槲皮素预孵育 30 分钟，然后在 37°C 下用 Dim 孵育 4 小时。
结果
体外孵育后，乐果在不同浓度下显著增加了丙二醛水平，显著降低了巯基水平，同时超氧化物歧化酶和过氧化氢酶活性显著增加。用槲皮素预处理的淋巴细胞对乐果诱导的研究参数的细胞毒性作用表现出显著的保护作用。
结论
综上所述，抗氧化剂槲皮素可通过降低脂质过氧化、蛋白质氧化，增加超氧化物歧化酶和过氧化氢酶活性，来减轻乐果诱导的人淋巴细胞氧化应激。
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