Qi Shun-Xiang, Han Guang-Yue, Liu Yan-Fang
Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050021, Hebei, China.
Zhongguo Yi Miao He Mian Yi. 2009 Feb;15(1):27-30.
To understand influenza B viruses circulating and antigenic variation and distributing in Hebei province during 2004-2008.
Viruses were isolated with Madin-Darby canine kidney (MDCK) cells. Viral RNA was extracted from isolates after serological identification and transcribed into cDNA by reverse transcript and ampified by PCR. The PCR product was purified and nucleotide sequence of HA1 gene of the influenza B viruses was analyzed.
131 influenza B viruses were obtained from October 2004 to March 2008. Among them, 48 strains were Victoria-lineage and 83 strains were Yamagata-lineage viruses. Yamagata-lineage viruses were detected in the 2004-2005, replaced by Victoria-lineage viruses in the 2005-2006. Both Victoria-lineage and Yamagata-lineage viruses were isolated in 2006-2007 and 2007-2008. There were several amino acids substitution in both Victoria and Yamagata lineages according to the result of sequence analysis of the hemagglutinin HA1 genes, and Yamagata lineages viruses took more frequently substitution in isolates. Influenza B viruses in 2008 were differed by 8 amino acids from virus stains, in 2005 with homology of 97.4%-98.0%.
The antigenicity of Victoria-lineage virus didn't take place varidation apparently in Hebei provinece during 2004-2008, but Yamagata-lineage virus took drift and caused outbreak of influenza in the epidemic season of 2007-2008.
了解2004 - 2008年河北省乙型流感病毒的流行、抗原变异及分布情况。
采用犬肾传代细胞（MDCK）分离病毒。血清学鉴定后从分离株中提取病毒RNA，经逆转录转录为cDNA，再通过PCR扩增。对PCR产物进行纯化，分析乙型流感病毒HA1基因的核苷酸序列。
2004年10月至2008年3月共获得131株乙型流感病毒。其中，48株为维多利亚系，83株为山形系病毒。2004 - 2005年检测到山形系病毒，2005 - 2006年被维多利亚系病毒取代。2006 - 2007年和2007 - 2008年同时分离到维多利亚系和山形系病毒。根据血凝素HA1基因序列分析结果，维多利亚系和山形系均有多个氨基酸替换，且山形系病毒在分离株中替换更为频繁。2008年的乙型流感病毒与2005年的病毒株相比，有8个氨基酸差异，同源性为97.4% - 98.0%。
2004 - 2008年河北省维多利亚系病毒抗原性未见明显变异，但山形系病毒发生了漂移，并在2007 - 2008年流行季节引发流感暴发。
