Zhou Jian-Hui, Wang Shuang, Chen Chao
Center for Disease Control and Prevention of Jilin Province, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Zhongguo Yi Miao He Mian Yi. 2009 Aug;15(4):310-5.
To establish a simple and quick method for identifying China vaccine strains and wild strains of Measles Virus.
To search the enzyme site in Hemagglutinin gene of measles virus for different domestic vaccine strains and wild strains of measles virus, and design the RT-PCR primer within the range covering the enzyme site, and then to confirm the specificity and sensibility of the RT-PCR method, and then identify the RT-PCR product by RFLP.
The one-step RT-PCR method is sensitive, the measles virus of 4.64 TCID50 can be detected at least. No positive bands can be found in the non-measles virus strains, it means that the RT-PCR method has good specificity, the PCR products of Chian measles vaccine strains of Shang-191 and Chang-47 were all cut into two fragments (287 bp and 151 bp) by Afi II, but two measles wild virus strains can't be cut by Afl II.
The RT-PCR-RFLP method which we established is a rapid and simple method for identifying China vaccine strain and wild strain.
建立一种简单快速的鉴别中国麻疹病毒疫苗株和野毒株的方法。
查找不同国产麻疹病毒疫苗株和野毒株血凝素基因中的酶切位点，在覆盖该酶切位点的范围内设计RT-PCR引物，然后验证RT-PCR方法的特异性和敏感性，再通过RFLP对RT-PCR产物进行鉴定。
一步法RT-PCR方法灵敏，至少能检测到4.64 TCID50的麻疹病毒。在非麻疹病毒株中未发现阳性条带，表明RT-PCR方法特异性良好，中国麻疹疫苗株沪191和长47的PCR产物经Afi II酶切均切成两个片段（287 bp和151 bp），但两株麻疹野病毒不能被Afl II酶切。
我们建立的RT-PCR-RFLP方法是一种快速简便的鉴别中国麻疹疫苗株和野毒株的方法。
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