Zhou Jian-Hui, Du Zhan-Sen, Chang Xin
Jilin Provincial Center for Disease Control and Prevention, Key Laboratory of Health Bureau of Jilin Province, Changchun 130062, Jilin, China.
Zhongguo Yi Miao He Mian Yi. 2009 Oct;15(5):417-22.
To study the genetic characteristic and amino acid mutation of hemogglutinin (H) protein of measles viruses circulated in Jilin provine in 2001-2008.
5 representative strains of measles virus circulated in Jilin provine in 2001-2008 were chosen to conduct the RNA extraction and the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for amplifying H gene and to do sequence analysis of the PCR products, and then to compare with Chinese vaccine strain S191 and 23 kinds of genotypes of measles virus representative strains from genebank for analysis of phylogenetic, nucleotide and amino acid sequence identity and variation, glycosylated domains variation.
5 strains of measles virus belong to H1 genotype, H1a sub-genotype. There were 6-17 (0.3%-0.9%) nucleotides and 3-9 (0.5%-1.5%) amino acids differences among 5 strains. There were 9-17 (0.5%-0.9%) nucleotides and 3-7 (0.5%-1.2%) amino acids differences between 5 strains and China 93-4, and 89-95 (4.8%-5.1%) nucleotides and 25-30 (4.1%-4.8%) amino acids differences between 5 strains and China vaccine S191. H protein of 5 measles virus all keep 4 glycosylation sites, the fifth glycosylation site NLS238-240 was losen because of the mutation from Set to Asn. in 240th amino acids.
5 measles virus circulated in Jilin province in 2001-2008 lose one glycosylation site which probably affected antigenencity. The nucleotides and amino acids differences among 5 strains were not too but both the nucleotides and amino acids identity showed falling trend year after year with both China 93-4 and S191. The H gene variation of measles virus in Jilin province was still increasing and accumulating year by year.
研究2001 - 2008年吉林省流行的麻疹病毒血凝素（H）蛋白的基因特征及氨基酸突变情况。
选取2001 - 2008年吉林省流行的5株代表性麻疹病毒株进行RNA提取及逆转录聚合酶链反应（RT-PCR）扩增H基因，对PCR产物进行序列分析，然后与中国疫苗株S191及基因库中23种麻疹病毒基因型代表性毒株进行比较，分析系统发育、核苷酸及氨基酸序列同一性及变异情况、糖基化结构域变异。
5株麻疹病毒属于H1基因型，H1a亚基因型。5株病毒之间存在6 - 17个（0.3% - 0.9%）核苷酸差异和3 - 9个（0.5% - 1.5%）氨基酸差异。5株病毒与中国93 - 4之间存在9 - 17个（0.5% - 0.9%）核苷酸差异和3 - 7个（0.5% - 1.2%）氨基酸差异，与中国疫苗株S191之间存在89 - 95个（4.8% - 5.1%）核苷酸差异和25 - 30个（4.1% - 4.8%）氨基酸差异。5株麻疹病毒H蛋白均保留4个糖基化位点，第5个糖基化位点NLS238 - 240因第240位氨基酸由Set突变为Asn而缺失。
2001 - 2008年吉林省流行的5株麻疹病毒缺失1个糖基化位点，可能影响抗原性。5株病毒之间核苷酸和氨基酸差异不大，但与中国93 - 4及S191相比，核苷酸和氨基酸同一性均呈逐年下降趋势。吉林省麻疹病毒H基因变异仍在逐年增加和积累。
