Mori T
Department of Virology, Kitasato Institute, Tokyo, Japan.
Biologicals. 1994 Jun;22(2):179-85. doi: 10.1006/biol.1994.1024.
Restriction fragment length polymorphism (RFLP) analysis was used to differentiate the AIK-C vaccine strain from the wild circulating measles viruses. Virus genomes were amplified by using reverse transcriptase-polymerase chain reaction (RT-PCR) in the part of the H-gene. The PCR product of the AIK-C strain was cut into 2 fragments after digestions with Hpall, BstPl and Avall, respectively. Otherwise, those of 17 wild-type strains isolated during 1950s-1991 were cleaved into 3 fragments only when digested with Avall. Comparison with wild strain sequences suggest that the above Hpall site and BstPl site are characteristic of the AIK-C strain. This molecular approach is also applied for the discrimination whether some symptoms after vaccination were related to the vaccine strain or not.
限制性片段长度多态性（RFLP）分析用于区分AIK-C疫苗株与野生传播的麻疹病毒。通过逆转录聚合酶链反应（RT-PCR）扩增病毒基因组的H基因部分。AIK-C株的PCR产物分别用HpaII、BstPI和AvaI消化后被切成2个片段。否则，1950年代至1991年期间分离的17株野生型毒株的PCR产物仅在用AvaI消化时被切成3个片段。与野生毒株序列的比较表明，上述HpaII位点和BstPI位点是AIK-C株的特征。这种分子方法也用于鉴别接种疫苗后的某些症状是否与疫苗株有关。
