Tao Ze-Xin, Li Yan, Wang Hai-Yan, Song Li-Zhi, Liu Gui-Fang, Liu Yao, Lin Xiao-Juan, Feng Lei, Yang He, Fan Qing-Ying, Xu Ai-Qiang
Shandong Center for Disease Control and Prevention, Jinan 250014, China.
Bing Du Xue Bao. 2009 Nov;25(6):410-4.
In order to study the genotypes and molecular evolution of human enterovirus (HEV) A species in Shandong Province, Stool samples were collected from AFP and HFMD patients in Shandong Province and virus isolation was performed. Reverse Transcription-Polymerase Chain Reactions (RT-PCR) specific for EV71 and CVA16 were performed with the virus isolates from HFMD patients. Positive isolates were selected for entire VP1 coding gene amplification and sequencing. Isolates with negative PCR results and isolates from AFP patients were selected for entire VP1 coding gene amplification and sequencing using primers specific for HEV A species. Phylogenetic tree was constructed among these VP1 nucleotide sequences and of other strains. Altogether 293 strains classified into 8 genotypes were isolated. The homologous comparison and phylogenetic analysis showed Shandong strains were distinct with prototype strains in every genotype. This report presents an overview of HEV-A in Shandong Province.
为研究山东省肠道病毒A种（HEV-A）的基因型及分子进化情况，采集了山东省急性弛缓性麻痹（AFP）和手足口病（HFMD）患者的粪便样本并进行病毒分离。对HFMD患者的病毒分离株进行了针对肠道病毒71型（EV71）和柯萨奇病毒A16型（CVA）的逆转录-聚合酶链反应（RT-PCR）。选择阳性分离株进行VP1编码基因全长扩增及测序。对PCR结果阴性的分离株以及AFP患者的分离株，使用针对HEV-A种的引物进行VP1编码基因全长扩增及测序。基于这些VP1核苷酸序列及其他毒株构建系统发育树。共分离出293株，分为8个基因型。同源性比较和系统发育分析表明，山东省分离株在每个基因型中均与原型株不同。本报告概述了山东省的HEV-A情况。
