Institute of Physical and Theoretical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Frankfurt am Main, Germany.
J Am Chem Soc. 2010 Feb 10;132(5):1454-5. doi: 10.1021/ja9077914.
Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4-U14, U4-U15, U14-U26, and U15-U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement.
脉冲电子双共振（PELDOR）光谱揭示了经过工程改造的 27 个核苷酸长的新霉素反应性核酶的预先排列的三级结构。在新霉素结合时，位于 U4-U14、U4-U15、U14-U26 和 U15-U26 位置的自旋标记之间的测量距离保持不变，这意味着在没有和存在配体的情况下，全局茎环结构得以保留。基于我们的结果，我们推断低温 PELDOR 数据明确证明了存在一组有利的焓构象，这些构象准备与配体结合，而无需进行主要的全局重排。
