Ge Huanhuan, Marchisio Mario Andrea
School of Pharmaceutical Science and Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, China.
Life (Basel). 2021 Mar 17;11(3):248. doi: 10.3390/life11030248.
Among noncoding RNA sequences, riboswitches and ribozymes have attracted the attention of the synthetic biology community as circuit components for translation regulation. When fused to aptamer sequences, ribozymes and riboswitches are enabled to interact with chemicals. Therefore, protein synthesis can be controlled at the mRNA level without the need for transcription factors. Potentially, the use of chemical-responsive ribozymes/riboswitches would drastically simplify the design of genetic circuits. In this review, we describe synthetic RNA structures that have been used so far in the yeast . We present their interaction mode with different chemicals (e.g., theophylline and antibiotics) or proteins (such as the RNase III) and their recent employment into clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas) systems. Particular attention is paid, throughout the whole paper, to their usage and performance into synthetic gene circuits.
在非编码RNA序列中，核糖开关和核酶作为翻译调控的电路元件，已引起合成生物学界的关注。当与适体序列融合时，核酶和核糖开关能够与化学物质相互作用。因此，无需转录因子即可在mRNA水平上控制蛋白质合成。化学响应性核酶/核糖开关的使用可能会极大地简化遗传电路的设计。在这篇综述中，我们描述了迄今为止在酵母中使用的合成RNA结构。我们展示了它们与不同化学物质（如茶碱和抗生素）或蛋白质（如核糖核酸酶III）的相互作用模式，以及它们最近在成簇规律间隔短回文重复序列-CRISPR相关蛋白9（CRISPR-Cas）系统中的应用。在整篇论文中，我们特别关注它们在合成基因电路中的使用和性能。
