Institute of Medical and Physiological Chemistry, Biomedical Center, University of Uppsala, Uppsala, Sweden.
Ups J Med Sci. 1977;82(3):167-81. doi: 10.3109/03009737709179097.
The implications of a double polar-nonpolar-polar leaflet construction of the plasma membrane are investigated. Experimental data from transmission electron microscopic and enzymologic characterization of plasma membranes are advantageously interpreted in these terms compared to interpretation in terms of lipid bilayer. X-ray diffraction and electron spin resonance studies do not differentiate between the present and previous models for the structure of plasma membranes but electron spin resonance data that fail to indicate a statistical distribution of spin labels also fail to support the fluid mosaic model for cell membranes. Results from experiments involving vectorial digestion and labelling of plasma membranes as well as freeze fracture electron microscopic data are compatible with the present model. The molecular composition of the human erythrocyte membrane is investigated whereby the band III protein and glycophorin are suggested to be the structural proteins of the outer leaflet and the spectrins those of the inner leaflet.
本文探讨了细胞膜双层极性-非极性-极性脂双层结构的含义。与脂质双层模型相比，利用透射电子显微镜和酶学特性对质膜进行的实验数据在这些方面得到了很好的解释。X 射线衍射和电子自旋共振研究不能区分目前的模型和以前的质膜结构模型，但电子自旋共振数据不能表明自旋标记的统计分布也不能支持细胞膜的流动镶嵌模型。涉及质膜定向消化和标记以及冷冻断裂电子显微镜数据的实验结果与目前的模型是一致的。本文还研究了人红细胞膜的分子组成，认为带 III 蛋白和血型糖蛋白是外叶的结构蛋白，血影蛋白是内叶的结构蛋白。
