Zhang Jing, Wu Ying-hui, Kong Hai-ying, Zhou Xiao-ge, Jin Ha-si, Wu Xiao-ming, Zhang Dan-dan, Gong Li-ping
Department of Pathology, Basic Medical College, Capital Medical University, Beijing 100069, China.
Zhonghua Bing Li Xue Za Zhi. 2009 Nov;38(11):739-44.
To evaluate the efficiency of the BIOMED-2 PCR assay and its implication in the diagnosis of mature B-cell non-Hodgkin's lymphomas.
Clinical, morphological and immunohistochemical features of 72 cases of non-Hodgkin's lymphomas were studied, including 25 reactive lymphoid hyperplasia, 37 diffuse large B cell lymphomas (DLBCL) and 35 extranodal marginal zone lymphomas of mucosa associated lymphoid tissues (MALT lymphoma and in addition, 25 cases of reactive lymphoid hyperplasia were used as the controls). DNA was exacted from the paraffin embedded formalin fixed tissue blocks and the quality of DNA was assessed using the BIOMED-2 specimen control reaction. Adequate samples were then analyzed by BIOMED-2 for immunoglobulin heavy and kappa light chain rearrangements.
Adequate DNA was obtained in 83 of 97 samples, including 60 mature B cell lymphomas and 23 reactive lymphoid hyperplasia. Clonal B-cell gene rearrangements were detected in 57 of 60 (95%) lymphomas. In contrast, clonal Ig gene rearrangements were not detected in any of the 23 cases of reactive lymphoid hyperplasia.
BIOMED-2 assay is highly sensitive and specific for the detection of clonal B cell gene rearrangement using routine paraffin embedded formalin fixed specimens.
评估BIOMED-2聚合酶链反应（PCR）检测方法的效率及其在成熟B细胞非霍奇金淋巴瘤诊断中的意义。
研究了72例非霍奇金淋巴瘤的临床、形态学和免疫组化特征，包括25例反应性淋巴组织增生、37例弥漫性大B细胞淋巴瘤（DLBCL）和35例黏膜相关淋巴组织结外边缘区淋巴瘤（MALT淋巴瘤），另外将25例反应性淋巴组织增生用作对照。从福尔马林固定石蜡包埋的组织块中提取DNA，并使用BIOMED-2样本对照反应评估DNA质量。然后用BIOMED-2对足够的样本进行免疫球蛋白重链和κ轻链重排分析。
97个样本中的83个获得了足够的DNA，包括60例成熟B细胞淋巴瘤和23例反应性淋巴组织增生。60例淋巴瘤中的57例（95%）检测到克隆性B细胞基因重排。相比之下，23例反应性淋巴组织增生中均未检测到克隆性Ig基因重排。
BIOMED-2检测方法使用常规福尔马林固定石蜡包埋标本检测克隆性B细胞基因重排具有高度敏感性和特异性。
