最可能数（MPN）-PCR 与实时 PCR 测量土壤中 alkB 同源基因丰度和多样性的比较研究。
A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.
机构信息
Technische Universität München, Chair of Soil Ecology, 85764 Neuherberg, Germany.
出版信息
J Microbiol Methods. 2010 Mar;80(3):295-8. doi: 10.1016/j.mimet.2010.01.005. Epub 2010 Jan 15.
PMID:20079768
Abstract
The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.
摘要
利用定量 PCR（qPCR）方法，如 MPN-qPCR 和实时-qPCR，对功能基因进行原位评估，会产生实质性的定量和定性差异。我们通过针对一组具有不同烃含量的环境样本中的与脂肪族烷烃生物降解相关的 alkB 基因，展示了这一点。
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