线粒体核糖体蛋白 S29（MRPS29）表达的 5' 上游开放阅读框调节。
Regulation of mitochondrial ribosomal protein S29 (MRPS29) expression by a 5'-upstream open reading frame.
机构信息
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
出版信息
Mitochondrion. 2010 Apr;10(3):274-83. doi: 10.1016/j.mito.2009.12.150. Epub 2010 Jan 14.
Abstract
Mitochondrial ribosomal protein S29 (MRPS29) is a mitochondrial pro-apoptotic protein also known as death associated protein 3 (DAP3). Over-expression of MRPS29 has been reported to induce apoptosis in several different human cell lines while conferring resistance in glioma and Ataxia telangiectasia cells. These two contradictory reports led us to investigate the MRPS29-induced apoptosis further. Cyber searches of the EST databases revealed the presence of a splice variant of MRPS29 mRNA containing an upstream open reading frame (uORF) at the 5' untranslated region (UTR). In this study, we confirmed the presence of this uORF using real-time RT-PCR and investigated its role in MRPS29 expression.
摘要
线粒体核糖体蛋白 S29（MRPS29）是一种线粒体促凋亡蛋白，也被称为凋亡相关蛋白 3（DAP3）。已有报道称，MRPS29 的过表达可诱导几种不同的人类细胞系发生凋亡，同时使神经胶质瘤和共济失调毛细血管扩张细胞产生抗性。这两个相互矛盾的报告促使我们进一步研究 MRPS29 诱导的凋亡。EST 数据库的网络搜索显示，MRPS29 mRNA 的剪接变体存在于 5'非翻译区（UTR）的上游开放阅读框（uORF）中。在本研究中，我们使用实时 RT-PCR 证实了该 uORF 的存在，并研究了其在 MRPS29 表达中的作用。
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